Unsal Funda, Sönmez Mehmet Fatih
Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Adv Clin Exp Med. 2014 May-Jun;23(3):363-70.
Ghrelin is a hormone which has effects on the secretion of growth hormone, the gastrointestinal system, the cardiovascular system, cell proliferation and the reproductive system.
The aim of this study is to investigate structural changes in the uterine tissue and to assess ghrelin immunoreactivity in the endometrium as a result of bilateral ovariectomization of rats.
In this study, 28 adult female albino Wistar rats were used. The rats were randomly divided into four groups. Group I was the control group; Group II was the placebo group; Group III was ovariectomized; and Group IV was ovariectomized with 2mg/kg estrogen administered per day. Age-matched diestrous intact rats were used as controls. At the end of the experiment, the rats were decapitated 1, 3, 5, 7, and 15 days after ovariectomy under ketamine anesthesia and their uterine tissue was removed.
In the ovariectomized rats, reductions in the sizes of both the uterine epithelium and the endometrial glands were observed, as well as a loss of connective tissue. Ghrelin-positive cells in the endometrial surface and the gland epithelium were visualized by immunohistochemistry. After ovariectomization, ghrelin expression was found to be decreased in a time dependent manner.
Bilateral ovariectomization leads to histological changes in the uterine tissue. Ovariectomization was observed to decrease endometrium ghrelin immunoreactivity.
胃饥饿素是一种对生长激素分泌、胃肠系统、心血管系统、细胞增殖及生殖系统有影响的激素。
本研究旨在探讨大鼠双侧卵巢切除术后子宫组织的结构变化，并评估子宫内膜中胃饥饿素的免疫反应性。
本研究使用了28只成年雌性白化Wistar大鼠。大鼠被随机分为四组。第一组为对照组；第二组为安慰剂组；第三组为卵巢切除组；第四组为卵巢切除且每天给予2mg/kg雌激素组。以年龄匹配的动情期完整大鼠作为对照。实验结束时，在氯胺酮麻醉下于卵巢切除术后1、3、5、7和15天对大鼠进行断头处理，并取出其子宫组织。
在卵巢切除的大鼠中，观察到子宫上皮和子宫内膜腺体大小减小，以及结缔组织丧失。通过免疫组织化学观察到子宫内膜表面和腺体上皮中的胃饥饿素阳性细胞。卵巢切除术后，发现胃饥饿素表达呈时间依赖性降低。
双侧卵巢切除术导致子宫组织发生组织学变化。观察到卵巢切除术降低了子宫内膜胃饥饿素免疫反应性。
