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用于埃及血吸虫病诊断的超低成本尿液过滤:一项概念验证研究。

Ultra-low-cost urine filtration for Schistosoma haematobium diagnosis: a proof-of-concept study.

作者信息

Ephraim Richard K D, Duah Evans, Andrews Jason R, Bogoch Isaac I

机构信息

Division of Medical Laboratory Technology, University of Cape Coast, Cape Coast, Ghana; Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California; Divisions of Internal Medicine and Infectious Diseases, University Health Network, Toronto, Ontario, Canada; Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.

Division of Medical Laboratory Technology, University of Cape Coast, Cape Coast, Ghana; Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California; Divisions of Internal Medicine and Infectious Diseases, University Health Network, Toronto, Ontario, Canada; Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

出版信息

Am J Trop Med Hyg. 2014 Sep;91(3):544-6. doi: 10.4269/ajtmh.14-0221. Epub 2014 Jun 30.

DOI:10.4269/ajtmh.14-0221
PMID:24980496
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4155557/
Abstract

Simple, efficient, and cost-effective strategies are needed for urine sample preparation in the field diagnosis of infection with Schistosoma haematobium. In this proof-of-concept study, we evaluated inexpensive and widely available paper products (paper towels, school workbook paper, and newspaper) to gravity-filter urine containing 60 eggs/mL of Schistosoma haematobium. Eggs were reliably visualized by light microscopy by using single-ply paper towels as urine filters. This filtration method has broad applicability in clinical and public health settings in resource-constrained environments.

摘要

在埃及血吸虫感染的现场诊断中,需要简单、高效且经济有效的尿液样本制备策略。在这项概念验证研究中,我们评估了价格低廉且广泛可得的纸制品(纸巾、学校练习册纸和报纸)对含有每毫升60个埃及血吸虫卵的尿液进行重力过滤的效果。通过使用单层纸巾作为尿液过滤器,卵可通过光学显微镜可靠地观察到。这种过滤方法在资源有限环境下的临床和公共卫生环境中具有广泛的适用性。

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