Bogoch Isaac I, Koydemir Hatice C, Tseng Derek, Ephraim Richard K D, Duah Evans, Tee Joseph, Andrews Jason R, Ozcan Aydogan
Divisions of General Internal Medicine and Infectious Diseases, University Health Network, Toronto, Ontario, Canada.
Department of Medicine, University of Toronto, Toronto, Ontario, Canada.
Am J Trop Med Hyg. 2017 Jun;96(6):1468-1471. doi: 10.4269/ajtmh.16-0912.
AbstractSchistosomiasis affects over 170 million people in Africa. Here we compare a novel, low-cost mobile phone microscope to a conventional light microscope for the label-free diagnosis of infections in a rural Ghanaian school setting. We tested the performance of our handheld microscope using 60 slides that were randomly chosen from an ongoing epidemiologic study in school-aged children. The mobile phone microscope had a sensitivity of 72.1% (95% confidence interval [CI]: 56.1-84.2), specificity of 100% (95% CI: 75.9-100), positive predictive value of 100% (95% CI: 86.3-100), and a negative predictive value of 57.1% (95% CI: 37.4-75.0). With its modest sensitivity and high specificity, this handheld and cost-effective mobile phone-based microscope is a stepping-stone toward developing a powerful tool in clinical and public health settings where there is limited access to conventional laboratory diagnostic support.
摘要
血吸虫病影响着非洲超过1.7亿人。在此，我们将一种新型低成本手机显微镜与传统光学显微镜进行比较，用于在加纳农村学校环境中对感染进行无标记诊断。我们使用从一项正在进行的学龄儿童流行病学研究中随机选取的60张载玻片测试了手持显微镜的性能。该手机显微镜的灵敏度为72.1%（95%置信区间[CI]：56.1 - 84.2），特异性为100%（95% CI：75.9 - 100），阳性预测值为100%（95% CI：86.3 - 100），阴性预测值为57.1%（95% CI：37.4 - 75.0）。这种手持且经济高效的手机显微镜具有适度的灵敏度和高特异性，是在传统实验室诊断支持有限的临床和公共卫生环境中开发强大工具的垫脚石。
