Genome-wide identification of CRISPR/Cas9 off-targets in human genome.
作者信息
Duan Jinzhi, Lu Guangqing, Xie Zhan, Lou Mingliang, Luo Jiao, Guo Lei, Zhang Yu
机构信息
1] Graduate Program, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China [2] National Institute of Biological Sciences, Beijing 102206, China.
National Institute of Biological Sciences, Beijing 102206, China.
出版信息
Cell Res. 2014 Aug;24(8):1009-12. doi: 10.1038/cr.2014.87. Epub 2014 Jul 1.
Abstract
摘要
相似文献
1
Genome-wide identification of CRISPR/Cas9 off-targets in human genome.人类基因组中CRISPR/Cas9脱靶效应的全基因组鉴定
Cell Res. 2014 Aug;24(8):1009-12. doi: 10.1038/cr.2014.87. Epub 2014 Jul 1.
2
CRISPR/Cas9-mediated genome editing of Epstein-Barr virus in human cells.CRISPR/Cas9介导的人类细胞中爱泼斯坦-巴尔病毒的基因组编辑。
J Gen Virol. 2015 Mar;96(Pt 3):626-636. doi: 10.1099/jgv.0.000012. Epub 2014 Dec 12.
3
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.具有不可检测的全基因组脱靶效应的高保真CRISPR-Cas9核酸酶。
Nature. 2016 Jan 28;529(7587):490-5. doi: 10.1038/nature16526. Epub 2016 Jan 6.
4
Recent Progress in CRISPR/Cas9 Technology.CRISPR/Cas9技术的最新进展
J Genet Genomics. 2016 Feb 20;43(2):63-75. doi: 10.1016/j.jgg.2016.01.001. Epub 2016 Jan 18.
5
Genome-wide target specificities of CRISPR RNA-guided programmable deaminases.CRISPR RNA 引导的可编程脱氨酶的全基因组靶向特异性。
Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10.
6
CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells.基于CRISPR的人类细胞中可控基因递送的自我切割机制。
Nucleic Acids Res. 2015 Jan;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
7
Genome editing: The domestication of Cas9.基因组编辑：Cas9的驯化
Nature. 2016 Jan 28;529(7587):468-9. doi: 10.1038/529468a.
8
Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3.对CRISPR相关核酸酶-解旋酶Cas3介导的DNA干扰的分子见解。
Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):16359-64. doi: 10.1073/pnas.1410806111. Epub 2014 Nov 3.
9
CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells.CRISPR/Cas9：活细胞中RNA成像的新工具。
Chembiochem. 2016 Sep 15;17(18):1682-4. doi: 10.1002/cbic.201600342. Epub 2016 Jul 22.
10
Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.工程化DNA结合分子如TAL蛋白和CRISPR/Cas系统在生物学研究中的应用
Int J Mol Sci. 2015 Sep 24;16(10):23143-64. doi: 10.3390/ijms161023143.
引用本文的文献
1
New hope and promise with CRISPR-Cas9 technology for the treatment of HIV.CRISPR-Cas9技术为治疗艾滋病带来新希望与前景。
Funct Integr Genomics. 2025 May 24;25(1):108. doi: 10.1007/s10142-025-01613-1.
2
RhoB regulates prostate cancer cell proliferation and docetaxel sensitivity via the PI3K-AKT signaling pathway.RhoB通过PI3K-AKT信号通路调节前列腺癌细胞的增殖和多西他赛敏感性。
BMC Cancer. 2025 Feb 26;25(1):354. doi: 10.1186/s12885-025-13762-4.
3
Progress and Challenges in HIV-1 Vaccine Research: A Comprehensive Overview.人类免疫缺陷病毒1型疫苗研究的进展与挑战：全面综述
Vaccines (Basel). 2025 Jan 31;13(2):148. doi: 10.3390/vaccines13020148.
4
Tracking-seq reveals the heterogeneity of off-target effects in CRISPR-Cas9-mediated genome editing.追踪测序揭示了CRISPR-Cas9介导的基因组编辑中脱靶效应的异质性。
Nat Biotechnol. 2025 May;43(5):799-810. doi: 10.1038/s41587-024-02307-y. Epub 2024 Jul 2.
5
Epigenetics of Early Cardiometabolic Disease: Mechanisms and Precision Medicine.早期心脏代谢疾病的表观遗传学：机制与精准医学。
Circ Res. 2023 Jun 9;132(12):1648-1662. doi: 10.1161/CIRCRESAHA.123.322135. Epub 2023 Jun 8.
6
Off-target effects in CRISPR/Cas9 gene editing.CRISPR/Cas9基因编辑中的脱靶效应。
Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157. eCollection 2023.
7
The impact of nucleosome structure on CRISPR/Cas9 fidelity.核小体结构对 CRISPR/Cas9 保真度的影响。
Nucleic Acids Res. 2023 Mar 21;51(5):2333-2344. doi: 10.1093/nar/gkad021.
8
Clinical application and detection techniques of liquid biopsy in gastric cancer.液体活检在胃癌中的临床应用及检测技术。
Mol Cancer. 2023 Jan 11;22(1):7. doi: 10.1186/s12943-023-01715-z.
9
CRISPR/Cas9: a tool to eradicate HIV-1.CRISPR/Cas9：一种清除 HIV-1 的工具。
AIDS Res Ther. 2022 Dec 1;19(1):58. doi: 10.1186/s12981-022-00483-y.
10
Chromatin as self-returning walks: From population to single cell and back.作为自我返回行走的染色质：从群体到单细胞再回归
Biophys Rep (N Y). 2021 Dec 10;2(1):100042. doi: 10.1016/j.bpr.2021.100042. eCollection 2022 Mar 9.
本文引用的文献
1
Crystal structure of Cas9 in complex with guide RNA and target DNA.Cas9 蛋白与向导 RNA 和靶 DNA 复合物的晶体结构
Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13.
2
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.Cas9 核酸内切酶的结构揭示了 RNA 介导的构象激活。
Science. 2014 Mar 14;343(6176):1247997. doi: 10.1126/science.1247997. Epub 2014 Feb 6.
3
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.CRISPR RNA 引导的内切酶 Cas9 对 DNA 的检测。
Nature. 2014 Mar 6;507(7490):62-7. doi: 10.1038/nature13011. Epub 2014 Jan 29.
4
A cut above the rest: targeted genome editing technologies in human pluripotent stem cells.青出于蓝而胜于蓝：人类多能干细胞中的靶向基因组编辑技术。
J Biol Chem. 2014 Feb 21;289(8):4594-9. doi: 10.1074/jbc.R113.488247. Epub 2013 Dec 20.
5
Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.利用优化的 CRISPR/Cas 系统在活人体细胞内对基因组位点进行动态成像。
Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
6
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.CRISPR/Cas 衍生的 RNA 引导内切酶和 Nickase 的脱靶效应分析。
Genome Res. 2014 Jan;24(1):132-41. doi: 10.1101/gr.162339.113. Epub 2013 Nov 19.
7
Cas9 as a versatile tool for engineering biology.Cas9 作为一种通用的基因编辑工具。
Nat Methods. 2013 Oct;10(10):957-63. doi: 10.1038/nmeth.2649.
8
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.高通量分析脱靶 DNA 切割揭示了 RNA 编程的 Cas9 核酸酶特异性。
Nat Biotechnol. 2013 Sep;31(9):839-43. doi: 10.1038/nbt.2673. Epub 2013 Aug 11.
9
DNA targeting specificity of RNA-guided Cas9 nucleases.RNA 引导的 Cas9 核酸酶的 DNA 靶向特异性。
Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647. Epub 2013 Jul 21.
10
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes.CRISPR 介导的真核生物模块化 RNA 引导的转录调控。
Cell. 2013 Jul 18;154(2):442-51. doi: 10.1016/j.cell.2013.06.044. Epub 2013 Jul 11.
Zotero 插件