Udou T, Mizuguchi Y, Wallace R J
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
FEMS Microbiol Lett. 1989 Jan 15;48(2):227-30. doi: 10.1111/j.1574-6968.1989.tb03304.x.
All the rapidly growing mycobacteria tested, Mycobacterium fortuitum complex, M. smegmatis, M. phlei, and M. vaccae, contained one of two characteristics, but were different from previously recognized aminoglycoside-acetyltransferases. The acetylation reaction of both the enzymes from M. fortuitum and Pseudomonas aeruginosa (3-N-acetyltransferase-III) with radiolabeled acetyl coenzyme A was inhibited severely by oxalacetate. It was suggested that the inhibitory effect of oxalacetate is due to the condensation reaction between oxalacetate and acetyl coenzyme A resulting in the generation of citrate.
所有测试的快速生长分枝杆菌，包括偶然分枝杆菌复合体、耻垢分枝杆菌、草分枝杆菌和母牛分枝杆菌，都具有两种特征之一，但与先前公认的氨基糖苷乙酰转移酶不同。来自偶然分枝杆菌和铜绿假单胞菌的两种酶（3-N-乙酰转移酶III）与放射性标记的乙酰辅酶A的乙酰化反应受到草酰乙酸的严重抑制。有人提出，草酰乙酸的抑制作用是由于草酰乙酸与乙酰辅酶A之间的缩合反应导致柠檬酸盐的生成。
