Dombeck Daniel, Tank David
Cold Spring Harb Protoc. 2014 Jul 1;2014(7):726-36. doi: 10.1101/pdb.top081810.
A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain's microcircuitry is connected to animal behavior.
本文介绍了一种对清醒且可移动的小鼠神经群体进行功能、细胞分辨率成像的方法。该方法基于使用球形跑步机、头部固定装置和运动校正软件，这些有助于使用固定直立双光子显微镜对清醒大脑进行神经成像。这些方法已被证明适用于广泛的脑区，并且应该有助于进一步加深我们对大脑微回路中神经元群体活动如何与动物行为相关联的理解。
