Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Evid Based Complement Alternat Med. 2014;2014:716509. doi: 10.1155/2014/716509. Epub 2014 May 28.
Tectorigenin (TTR) is an O-methylated isoflavone derived from the rhizome of Belamacanda chinensis (L.) DC. It is known to perform a wide spectrum of biological activities such as antioxidant, anti-inflammatory, anti-tumor. The aim of this study is to examine the mechanism of antibacterial activity of TTR against methicillin-resistant Staphylococcus aureus (MRSA). The anti-MRSA activity of TTR was analyzed in combination assays with detergent, ATPase inhibitors, and peptidoglycan (PGN) derived from S. aureus. Transmission electron microscopy (TEM) was used to monitor survival characteristics and changes in S. aureus morphology. The MIC values of TTR against all the tested strains were 125 μ g/mL. The OD(600) of each suspension treated with a combination of Triton X-100, DCCD, and NaN3 with TTR (1/10 × MIC) had been reduced from 68% to 80%, compared to the TTR alone. At a concentration of 125 μ g/mL, PGN blocked antibacterial activity of TTR. This study indicates that anti-MRSA action of TTR is closely related to cytoplasmic membrane permeability and ABC transporter, and PGN at 125 μ g/mL directly bind to and inhibit TTR at 62.5 μ g/mL. These results can be important indication in study on antimicrobial activity mechanism against multidrug resistant strains.
蓬子菜苷（TTR）是一种来源于鸢尾科植物蓬子菜的 O-甲基异黄酮。它具有广泛的生物活性，如抗氧化、抗炎、抗肿瘤。本研究旨在探讨 TTR 对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性机制。TTR 对 MRSA 的抗活性与去污剂、ATP 酶抑制剂和来自金黄色葡萄球菌的肽聚糖（PGN）联合分析。透射电子显微镜（TEM）用于监测金黄色葡萄球菌的存活特征和形态变化。TTR 对所有测试菌株的 MIC 值均为 125μ g/mL。与单独使用 TTR 相比，用 Triton X-100、DCCD 和 NaN3 与 TTR（1/10×MIC）组合处理的每个悬浮液的 OD（600）从 68%降低到 80%。在 125μ g/mL 的浓度下，PGN 阻断了 TTR 的抗菌活性。本研究表明，TTR 对 MRSA 的作用与细胞质膜通透性和 ABC 转运体密切相关，125μ g/mL 的 PGN 直接结合并抑制 62.5μ g/mL 的 TTR。这些结果可为研究针对多药耐药菌株的抗菌活性机制提供重要依据。
