蜡样芽孢杆菌β-内酰胺酶II的硫代-β-内酰胺底物。必需金属离子与底物之间直接相互作用的证据。
A thiono-beta-lactam substrate for the beta-lactamase II of Bacillus cereus. Evidence for direct interaction between the essential metal ion and substrate.
作者信息
Murphy B P, Pratt R F
机构信息
Department of Chemistry, Wesleyan University, Middletown, CT 06457.
出版信息
Biochem J. 1989 Mar 15;258(3):765-8. doi: 10.1042/bj2580765.
Abstract
An 8-thionocephalosporin was shown to be a substrate of the beta-lactamase II of Bacillus cereus, a zinc metalloenzyme. Although it is a poorer substrate, as judged by the Kcat./Km parameter, than the corresponding 8-oxocephalosporin, the discrimination against sulphur decreased when the bivalent metal ion in the enzyme active site was varied in the order Mn2+ (the manganese enzyme catalysed the hydrolysis of the oxo compound but not that of the thiono compound), Zn2+, Co2+ and Cd2+. This result is taken as evidence for kinetically significant direct contact between the active-site metal ion of beta-lactamase II and the beta-lactam carbonyl heteroatom. No evidence was obtained, however, for accumulation of an intermediate with such co-ordination present.
摘要
一种8-硫代头孢菌素被证明是蜡状芽孢杆菌β-内酰胺酶II（一种锌金属酶）的底物。尽管根据催化常数/米氏常数参数判断，它是比相应的8-氧代头孢菌素更差的底物，但当酶活性位点中的二价金属离子按Mn2+（锰酶催化氧代化合物的水解但不催化硫代化合物的水解）、Zn2+、Co2+和Cd2+的顺序变化时，对硫的歧视性降低。该结果被视为β-内酰胺酶II活性位点金属离子与β-内酰胺羰基杂原子之间存在动力学上显著直接接触的证据。然而，没有获得存在这种配位中间体积累的证据。
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