Department of Polymer Science, The University of Akron, Akron, OH 44325-3909, USA.
Chem Soc Rev. 2014;43(20):7013-39. doi: 10.1039/c4cs00139g. Epub 2014 Jul 4.
Peptides that comprise the functional subunits of proteins have been conjugated to versatile materials (biomolecules, polymers, surfaces and nanoparticles) in an effort to modulate cell responses, specific binding affinity and/or self-assembly behavior. However, the efficient and convenient synthesis of peptide-conjugates, especially the constructs with multiple types of peptide functionality remains challenging. In this critical review, we focus on "click" reactions that have been used to synthesis peptide-functionalized conjugates, introducing their reaction conditions, specifically elucidating parameters that influence reaction kinetics and total conversion, and highlighting examples that have been completed recently. Moreover, orthogonal "click" reactions that synthesize multi-functional biomaterials in a one-pot or sequential manner are noted. Through this review, a comprehensive understanding of "click" reactions aims to provide insight on how one might choose suitable "click" reactions to constitute peptide-functionalized molecules/surfaces/matrices for the development of advanced biomaterials.
已经将构成蛋白质功能亚单位的肽与各种材料（生物分子、聚合物、表面和纳米粒子）连接起来，以调节细胞反应、特定的结合亲和力和/或自组装行为。然而，肽缀合物的高效和方便合成，特别是具有多种肽功能的构建物仍然具有挑战性。在这篇评论中，我们重点介绍了用于合成肽功能化缀合物的“点击”反应，介绍了它们的反应条件，特别是阐明了影响反应动力学和总转化率的参数，并强调了最近完成的例子。此外，还指出了可以一锅法或顺序法合成多功能生物材料的正交“点击”反应。通过这篇综述，全面了解“点击”反应有助于了解如何选择合适的“点击”反应来构成肽功能化分子/表面/基质，以开发先进的生物材料。
