Division of Reproductive Endocrinology and Infertility, Carolinas Healthcare System, Charlotte, North Carolina; Department of Obstetrics and Gynecology, Carolinas Healthcare System, Charlotte, North Carolina.
Department of Obstetrics and Gynecology, Carolinas Healthcare System, Charlotte, North Carolina.
Fertil Steril. 2014 Sep;102(3):705-10. doi: 10.1016/j.fertnstert.2014.05.039. Epub 2014 Jun 30.
To determine whether the period of ejaculatory abstinence (EA) influences the total antioxidant capacity (TAC) of semen or lipid peroxidation (LPO) of sperm membranes.
A prospective experimental trial.
Academic medical center for reproductive endocrinology and infertility.
PATIENT(S): Forty men from infertile couples planning intrauterine insemination.
INTERVENTION(S): Men provided semen specimens after EA periods of 1 and 4 days.
MAIN OUTCOME MEASURE(S): Semen analysis, peroxidase staining, and assays for seminal TAC and sperm membrane LPO, with measures compared between days 1 and 4 within individuals (internal control) using paired t tests.
RESULT(S): The shorter period of EA (1 day vs. 4 days) resulted in statistically significant decreases in semen volume (-24%), sperm density (-28%), and total sperm count (-3.2%). There was a statistically significant increase in TAC with the shorter period of EA (1 day) compared with 4 days of EA. No difference was detected in sperm membrane LPO comparing 1 day of EA and 4 days of EA.
CONCLUSION(S): Higher seminal TAC obtained after a shorter period of EA could diminish oxidative stress-induced sperm damage by a mechanism independent of LPO. Shorter periods of EA may thus improve sperm quality by protecting from reactive oxygen species damage, even though lower numbers of motile sperm are produced after a shorter period of EA. This would be consistent with prior research indicating improved results after intrauterine insemination under these circumstances.
确定射精禁欲期（EA）是否会影响精液的总抗氧化能力（TAC）或精子膜的脂质过氧化（LPO）。
前瞻性实验研究。
生殖内分泌学和不孕症的学术医疗中心。
计划宫内授精的不育夫妇中的 40 名男性。
男性在 EA 1 天和 4 天后提供精液样本。
精液分析、过氧化物酶染色以及 TAC 和精子膜 LPO 的测定，使用配对 t 检验在个体内（内部对照）比较 1 天和 4 天之间的测量值。
较短的 EA 期（1 天与 4 天）导致精液量（-24%）、精子密度（-28%）和总精子计数（-3.2%）的统计学显著下降。与 4 天的 EA 相比，较短的 EA 期（1 天）导致 TAC 统计学显著增加。在比较 1 天和 4 天的 EA 时，未发现精子膜 LPO 的差异。
较短的 EA 期后获得的较高精液 TAC 可以通过独立于 LPO 的机制减少氧化应激诱导的精子损伤。因此，较短的 EA 期可能通过保护精子免受活性氧损伤来改善精子质量，尽管在较短的 EA 期后产生的活动精子数量较少。这与先前的研究结果一致，表明在这种情况下进行宫内授精会取得更好的效果。
