母体蛋白质限制对大鼠后代肝脏代谢的影响。
Effect of maternal protein restriction on liver metabolism in rat offspring.
作者信息
Moraes Camila, Rebelato Hércules J, Amaral Maria Esmeria C, Resende Thais Marangoni, Silva Eduarda V C, Esquisatto Marcelo A M, Catisti Rosana
机构信息
Biomedical Sciences Graduate Program, Centro Universitário Hermínio Ometto, Uniararas, Av Maximiliano Baruto 500, 13607-339, Araras, SP, Brazil.
出版信息
J Physiol Sci. 2014 Sep;64(5):347-55. doi: 10.1007/s12576-014-0325-8. Epub 2014 Jul 4.
Abstract
Consequences of gestational protein restriction (GPR) on liver metabolism in rat offspring were investigated. Pregnant dams were divided into groups: normal (NP, 17% casein) or low-protein diet (LP, 6% casein). Livers were collected from 30-day-old offspring (d30) for analysis or isolation of mitochondria. At d30, hepatic and muscle glycogen was increased in LP group. Mitochondrial swelling and oxygen uptake (recorded with a Clark-type electrode) were significantly reduced in NP female and LP pups. Thiobarbituric acid reactive substances production was lower in females (NP or LP), suggesting significant inhibition of lipid peroxidation. Measurement of mitochondrial respiration (states 3 and 4 stimulated by succinate) showed a higher ADP/O ratio in LP pups, particularly females, suggesting higher phosphorylation efficiency. In the 1st month of life, under our experimental conditions, GPR protects liver mitochondria against oxidative stress and females seem to be more resistant or more suitable for survival.
摘要
研究了孕期蛋白质限制（GPR）对大鼠后代肝脏代谢的影响。将怀孕的母鼠分为两组：正常组（NP，17%酪蛋白）或低蛋白饮食组（LP，6%酪蛋白）。从30日龄的后代（d30）收集肝脏用于分析或分离线粒体。在d30时，LP组的肝脏和肌肉糖原增加。NP雌性和LP幼崽的线粒体肿胀和氧气摄取（用克拉克型电极记录）显著降低。雌性（NP或LP）的硫代巴比妥酸反应性物质产量较低，表明脂质过氧化受到显著抑制。线粒体呼吸测量（由琥珀酸刺激的状态3和状态4）显示LP幼崽，尤其是雌性，具有更高的ADP/O比值，表明磷酸化效率更高。在生命的第一个月，在我们的实验条件下，GPR可保护肝脏线粒体免受氧化应激，雌性似乎更具抵抗力或更适合生存。
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