Department of Biological Sciences and Bioengineering, Inha University, Incheon, 22212, Republic of Korea.
Department of Biological Sciences, Center for Molecular and Cell Biology, Inha University, Incheon, 22212, Republic of Korea.
J Microbiol. 2022 Aug;60(8):806-813. doi: 10.1007/s12275-022-2298-2. Epub 2022 Jul 29.
Two Gram-stain-positive, aerobic, chemoheterotrophic, non-motile, rod-shaped, and yellow-pigmented bacterial strains, designated IMCC34837 and IMCC34838, were isolated from a freshwater stream. Results of 16S rRNA gene-based phylogenetic analyses showed that strains IMCC34837 and IMCC34838 shared 96.3% sequence similarity and were most closely related to Flavihumibacter profundi Chu64-6-1 (99.6%) and Flavihumibacter cheonanensis WS16 (96.4%), respectively. Complete whole-genome sequences of strains IMCC34837 and IMCC34838 were 5.0 Mbp and 4.3 Mbp of genome size with 44.5% and 47.9% of DNA G + C contents, respectively. Average nucleotide identity (ANI) and digital DNA- DNA hybridization (dDDH) values between the two strains were 70.0% and 17.9%, repectively, revealing that they are independent species. The two strains showed ≤ 75.2% ANI and ≤ 19.3% dDDH values to each closely related species of the genus Flavihumibacter, indicating that the two strains represent each novel species. Major fatty acid constituents of strain IMCC34837 were iso-C, iso-C G and anteiso-C and those of strain IMCC34838 were iso-C and iso-C G. The predominant isoprenoid quinone detected in both strains was menaquinone-7 (MK-7). Major polar lipids of both strains were phosphatidylethanolamine, aminolipids, and glycolipids. Based on the phylogenetic and phenotypic characterization, strains IMCC34837 and IMCC34838 were considered to represent two novel species within the genus Flavihumibacter, for which the names Flavihumibacter fluminis sp. nov. and Flavihumibacter rivuli sp. nov. are proposed with IMCC34837 (= KACC 21752 = NBRC 115292) and IMCC34838 (= KACC 21753 = NBRC 115293) as the type strains, respectively.
两株革兰氏阳性、需氧、化能异养、非运动、杆状、呈黄色的细菌菌株，分别命名为 IMCC34837 和 IMCC34838，从一条淡水溪流中分离得到。基于 16S rRNA 基因的系统发育分析结果表明，菌株 IMCC34837 和 IMCC34838 的序列相似度为 96.3%，与 Flavihumibacter profundi Chu64-6-1（99.6%）和 Flavihumibacter cheonanensis WS16（96.4%）最为密切相关。菌株 IMCC34837 和 IMCC34838 的全基因组序列分别为 5.0 Mbp 和 4.3 Mbp，基因组大小分别为 44.5%和 47.9%的 DNA G+C 含量。两株菌之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 70.0%和 17.9%，表明它们是独立的种。两株菌与属内亲缘关系密切的种的 ANI 值均小于 75.2%，dDDH 值均小于 19.3%，表明两株菌分别代表两个新种。菌株 IMCC34837 的主要脂肪酸成分为 iso-C、iso-C G 和 anteiso-C，菌株 IMCC34838 的主要脂肪酸成分为 iso-C 和 iso-C G。两株菌中检测到的主要类异戊二烯醌均为menaquinone-7（MK-7）。两株菌的主要极性脂均为磷脂酰乙醇胺、氨基脂和糖脂。基于系统发育和表型特征，菌株 IMCC34837 和 IMCC34838 被认为代表黄杆菌属中的两个新种，分别命名为 Flavihumibacter fluminis sp. nov. 和 Flavihumibacter rivuli sp. nov.，其模式菌株分别为 IMCC34837（=KACC 21752=NBRC 115292）和 IMCC34838（=KACC 21753=NBRC 115293）。
