Lee Siwon, Lee Wonseok, Chung Hyen-Mi, Park Sangjung
Water Supply and Sewerage Research Division, National Institute of Environmental Research, Incheon, 22689, Republic of Korea.
Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon, 22689, Republic of Korea.
J Microbiol. 2017 Jun;55(6):417-420. doi: 10.1007/s12275-017-6313-y. Epub 2017 Apr 20.
A bacterial strain PBT33-2 was isolated from the air environment in an indoor pig farm. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PBT33-2 belonged to the genus Nocardioides in the phylum Actinobacteria, and was most closely related to Nocardioides daphnia D287 in a maximum-likelihood and neighbor-joining phylogenetic trees. Strain PBT33-2 shared 95.3% sequence identity with N. daphnia D287. However, the highest sequence similarity was shown with N. sediminis MSL-01 (96.0%). It had less than 96.0% sequence identities with other type species of the genus Nocardioides. Strain PBT-33-2 grew at 15-45°C (optimum 20-35°C), pH 5.0-11.0 (optimum pH 7.0) and 0-4.0% (w/v) NaCl (optimum 0%). The major fatty acid and quinone were iso-C and MK-8, and the DNA G+C content of strain PBT33-2 was 69.3 mol%. On the basis of poly-phasic results, strain PBT33-2 represents a novel species of the genus Nocardioides, for which the name Nocardioides suum sp. nov. is proposed. Its type strain is PBT33-2 (=KCTC 39558 =DSM 102833).
从室内养猪场的空气环境中分离出一株细菌PBT33-2。基于16S rRNA基因序列的系统发育分析表明，菌株PBT33-2属于放线菌门的诺卡氏菌属，在最大似然法和邻接法系统发育树中与达氏诺卡氏菌D287关系最为密切。菌株PBT33-2与达氏诺卡氏菌D287的序列同一性为95.3%。然而，与沉积物诺卡氏菌MSL-01的序列相似性最高（96.0%）。它与诺卡氏菌属的其他模式种的序列同一性小于96.0%。菌株PBT-33-2在15-45°C（最适温度20-35°C）、pH 5.0-11.0（最适pH 7.0）和0-4.0%（w/v）NaCl（最适0%）条件下生长。主要脂肪酸和醌分别为异-C和MK-8，菌株PBT33-2的DNA G+C含量为69.3 mol%。基于多相分类结果，菌株PBT33-2代表诺卡氏菌属的一个新物种，为此提出新名称猪诺卡氏菌（Nocardioides suum sp. nov.）。其模式菌株为PBT33-2（=KCTC 39558 =DSM 102833）。
