Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5439-5444. doi: 10.1099/ijsem.0.004435. Epub 2020 Sep 4.
A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48 was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48 was closely related to the genus , with 97.0 and 96.7 % sequence similarities to EBR4-1-2 and H5, respectively. Strain H23T48 possessed MK-9(H) as the major menaquinone and C (42.4 %), C 9 (31.3 %) and C (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine. The genomic G+C content of strain H23T48 is 59.5 mol% and the average nucleotide identity value between H23T48 and KCT C33148 (=EBR4-1-2) is 75.5 %. Based on the obtained data, strain H23T48 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H23T48 (=KCTC 49253=JCM 33282).
一株新型革兰氏阳性、非运动、非孢子形成、短杆状、严格需氧的细菌，被命名为 H23T48 菌株，从韩国首尔大公园动物园采集的东方鹳粪便样本中分离得到。H23T48 菌株的最佳生长条件为 30-37°C、pH8 和 3%（w/v）NaCl。基于 16S rRNA 基因序列的系统发育分析表明，H23T48 菌株与属密切相关，与 EBR4-1-2 和 H5 的序列相似度分别为 97.0%和 96.7%。H23T48 菌株含有 MK-9(H)作为主要的menaquinone 和 C (42.4%)、C 9 (31.3%)和 C 17.7%作为主要的细胞脂肪酸。极性脂包括磷脂酰甘油、两种未鉴定的脂类、六种未鉴定的磷脂和两种未鉴定的糖脂。细胞壁肽聚糖的氨基酸组成是 l-丙氨酸、l-赖氨酸、d-谷氨酸、l-天门冬氨酸和甘氨酸。H23T48 菌株的基因组 G+C 含量为 59.5mol%，H23T48 与 KCT C33148（=EBR4-1-2）之间的平均核苷酸同一性值为 75.5%。基于获得的数据，H23T48 菌株代表了属的一个新型物种，提议使用 sp. nov. 作为其名称。其模式菌株为 H23T48（=KCTC 49253=JCM 33282）。
