Mercolino T J, Locke A L, Afshari A, Sasser D, Travis W W, Arnold L W, Haughton G
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
J Exp Med. 1989 Jun 1;169(6):1869-77. doi: 10.1084/jem.169.6.1869.
5-15% of lymphocytes in the peritoneums of normal adult B10.H-2aH-4bp/Wts (2a4b) mice are CD5+ (Ly-1) B cells that recognize phosphatidyl choline (PtC), a phospholipid component of all mammalian cells. We produced a set of IgM-secreting hybridomas from the peritoneal cells of normal, adult 2a4b mice. We found that this set of hybridomas shows a similarly high frequency of antibodies specific for PtC (21 of 86) that also react with bromelain-treated mouse erythrocytes. Restriction fragment analysis of Ig gene rearrangements and analysis of expressed Ig idiotypes reveal that these cells use a restricted set of variable region genes to generate the PtC-specific antibodies. The Ig genes used by the PtC-specific hybridomas appear to be the same as those found in the PtC-specific Ly-1 B cell lymphomas, CH27 and CH34.
在正常成年B10.H-2aH-4bp/Wts（2a4b）小鼠的腹膜中，15%的淋巴细胞是CD5+（Ly-1）B细胞，这些细胞能够识别磷脂酰胆碱（PtC），即所有哺乳动物细胞的一种磷脂成分。我们从正常成年2a4b小鼠的腹膜细胞中制备了一组分泌IgM的杂交瘤。我们发现，这组杂交瘤中对PtC具有特异性的抗体频率同样很高（86个中有21个），这些抗体也能与菠萝蛋白酶处理过的小鼠红细胞发生反应。对Ig基因重排的限制性片段分析以及对表达的Ig独特型的分析表明，这些细胞利用一组有限的可变区基因来产生针对PtC的特异性抗体。PtC特异性杂交瘤所使用的Ig基因似乎与在PtC特异性Ly-1 B细胞淋巴瘤CH27和CH34中发现的基因相同。
