Division of Genetics and Molecular Biology, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
Sensors (Basel). 2012;12(4):4016-30. doi: 10.3390/s120404016. Epub 2012 Mar 27.
Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-L-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.
群体感应控制着大多数变形菌的毒力决定因素。在这项工作中，一种阿育吠陀香料丁香（Syzygium aromaticum）的己烷、氯仿和甲醇提取物显示出抗群体感应活性。丁香的己烷和甲醇提取物抑制了 C. violaceum CV026 对外源提供的 N-己酰基高丝氨酸内酯的反应，从而阻止了紫胶红的产生。丁香的氯仿和甲醇提取物显著减少了在 N-（3-氧代十二酰基）-L-高丝氨酸内酯存在下生长的大肠杆菌 [pSB1075] 的生物发光产物。我们证明丁香提取物抑制了铜绿假单胞菌 PA01 的群体感应调节表型，包括 lecA::lux 的表达（己烷提取物）、群集（甲醇提取物最大抑制）、绿脓菌素（己烷提取物最大抑制）。这项研究表明，丁香提取物中存在具有抗群体感应活性的天然化合物，可能可作为抗感染药物的先导。
