Department of Pharmacy, First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, Liaoning Province, People's Republic of China.
Inflammation. 2015 Apr;38(2):623-31. doi: 10.1007/s10753-014-9969-z.
Arctigenin (ATG) has been reported to possess anti-inflammatory properties. However, the effects of ATG on lipopolysaccharide (LPS)-induced acute lung injury (ALI) remains not well understood. In the present study, our investigation was designed to reveal the effect of ATG on LPS-induced ALI in rats. We found that ATG pretreatment attenuated the LPS-induced ALI, as evidenced by the reduced histological scores, myeloperoxidase activity, and wet-to-dry weight ratio in the lung tissues. This was accompanied by the decreased levels of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-1 (IL-6) in the bronchoalveolar lavage fluid. Furthermore, ATG downregulated the expression of nuclear factor kappa B (NF-κB) p65, promoted the phosphorylation of inhibitor of nuclear factor-κB-α (IκBα) and activated the adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPKα) in the lung tissues. Our results suggested that ATG attenuates the LPS-induced ALI via activation of AMPK and suppression of NF-κB signaling pathway.
白花前胡甲素 (ATG) 已被报道具有抗炎作用。然而，ATG 对脂多糖 (LPS) 诱导的急性肺损伤 (ALI) 的影响尚不清楚。在本研究中，我们旨在研究 ATG 对 LPS 诱导的大鼠 ALI 的影响。我们发现，ATG 预处理可减轻 LPS 诱导的 ALI，这表现在肺组织的组织学评分、髓过氧化物酶活性和湿重/干重比降低。这伴随着支气管肺泡灌洗液中肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β) 和白细胞介素-1 (IL-6) 的水平降低。此外，ATG 下调了核因子-κB (NF-κB) p65 的表达，促进了核因子-κB-α (IκBα) 的磷酸化，并激活了肺组织中的腺苷 5'-单磷酸 (AMP)-激活的蛋白激酶 (AMPKα)。我们的结果表明，ATG 通过激活 AMPK 和抑制 NF-κB 信号通路来减轻 LPS 诱导的 ALI。
