Yusuf E, Van Der Meeren S, Schallier A, Piérard D
Department of Medical Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium,
Eur J Clin Microbiol Infect Dis. 2014 Dec;33(12):2237-40. doi: 10.1007/s10096-014-2199-3. Epub 2014 Jul 10.
The purpose of this investigation was to compare the performance and cost of the Carba NP test with the Rapid CARB Screen Kit in detecting the presence of carbapenemase in Enterobacteriaceae and Pseudomonas aeruginosa. Ninety-two Enterobacteriaceae and 19 P. aeruginosa strains were used in this study. Multiplex polymerase chain reaction (PCR) was performed to determine whether these microorganisms harboured bla VIM, bla IMP, bla NDM, bla KPC and bla OXA-48. The Carba NP test and Rapid CARB Screen Kit were used on the strains according to the standardised protocols. The sensitivity, specificity and positive and negative predictive values of the tests were calculated. The cost of performing one test was also calculated. Forty-five Enterobacteriaceae and six P. aeruginosa were found to harbour carbapenemase-encoding genes. The Carba NP test had sensitivities of 91.1 % and 100 % for Enterobacteriaceae and P. aeruginosa, respectively. The Rapid CARB Screen Kit had sensitivities of 73.3 % and 66.7 % for Enterobacteriaceae and P. aeruginosa, respectively. The specificity of both tests was 100 %. The approximated price for performing one Carba NP test was 0.31 Euros and for CARB Screen Kit, it was 1.25 Euros. The Carba NP test performed better than the Rapid CARB Screen Kit in detecting carbapenemase production in Enterobacteriaceae and P. aeruginosa. The cost to perform both tests is reasonable.
本研究的目的是比较Carba NP试验与快速碳青霉烯酶检测试剂盒在检测肠杆菌科细菌和铜绿假单胞菌中碳青霉烯酶方面的性能和成本。本研究使用了92株肠杆菌科细菌和19株铜绿假单胞菌。采用多重聚合酶链反应（PCR）来确定这些微生物是否携带bla VIM、bla IMP、bla NDM、bla KPC和bla OXA - 48。按照标准化方案对这些菌株进行Carba NP试验和快速碳青霉烯酶检测试剂盒检测。计算了试验的敏感性、特异性以及阳性和阴性预测值。还计算了进行一次试验的成本。发现45株肠杆菌科细菌和6株铜绿假单胞菌携带碳青霉烯酶编码基因。Carba NP试验对肠杆菌科细菌和铜绿假单胞菌的敏感性分别为91.1%和100%。快速碳青霉烯酶检测试剂盒对肠杆菌科细菌和铜绿假单胞菌的敏感性分别为73.3%和66.7%。两种试验的特异性均为100%。进行一次Carba NP试验的近似价格为0.31欧元，快速碳青霉烯酶检测试剂盒为1.25欧元。在检测肠杆菌科细菌和铜绿假单胞菌中的碳青霉烯酶产生方面，Carba NP试验比快速碳青霉烯酶检测试剂盒表现更好。进行这两种试验的成本是合理的。
