大马士革蔷薇水醇提取物对大鼠回肠收缩的体外影响及其作用机制

In Vitro Impact of Hydro-alcoholic Extract of Rosa damascena Mill. on Rat Ileum Contractions and the Mechanisms Involved.

作者信息

Sedighi Mehrnoosh, Rafieian-Kopaei Mahmoud, Noori-Ahmadabadi Mosayeb, Godarzi Iraj, Baradaran Azar

机构信息

Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Int J Prev Med. 2014 Jun;5(6):767-75.

BACKGROUND

The petal's hydro-alcoholic extract of Rosa damascena Mill. on ileum contractions of Wistar rats and its possible mechanism were investigated.

METHODS

Forty-eight male Wistar rats were divided into six groups. Ileum was placed adjacent to propranolol (1 μM), naloxone (1 μM) and L-NAME (100 μM) and also under the influence of different doses (2-8 mM) of calcium chloride.

RESULTS

Cumulative extract of R. damascena Mill. (100, 500, and 1000 mg/L) decreased ileum contractions induced by KCl (60 mM) in a dose-dependent manner (P < 0.0001). Propranolol and naloxone significantly decreased the inhibitory effect of the extract on contractions induced by KCl (P < 0.001), but L-NAME was ineffective. Furthermore, calcium led to the contraction of depolarized tissue through KCI and this contractile effect decreased significantly induced by the cumulative concentrations of the extract (P < 0.001).

CONCLUSIONS

The results indicate that R. damascena Mill. dose-dependently (100, 500, and 1000 mg/L) decreases ileum movements of the rat probably through stimulating the β-adrenergic and opioid receptors and voltage-dependent calcium channels, and it may be used to treat digestive disorders.

背景

研究了大马士革蔷薇花瓣的水醇提取物对Wistar大鼠回肠收缩的影响及其可能机制。

方法

将48只雄性Wistar大鼠分为六组。回肠分别置于普萘洛尔（1 μM）、纳洛酮（1 μM）和L-精氨酸甲酯（100 μM）附近，并在不同剂量（2-8 mM）氯化钙的影响下。

结果

大马士革蔷薇累积提取物（100、500和1000 mg/L）以剂量依赖性方式降低了由氯化钾（60 mM）诱导的回肠收缩（P < 0.0001）。普萘洛尔和纳洛酮显著降低了提取物对氯化钾诱导的收缩的抑制作用（P < 0.001），但L-精氨酸甲酯无效。此外，钙通过氯化钾导致去极化组织收缩，而提取物的累积浓度显著降低了这种收缩作用（P < 0.001）。