Gulaya N M, Volkov G L, Klimashevsky V M, Govseeva N M, Artemenko I P, Gavriliuk E S
A. V. Palladine Institute of Biochemistry, Ukrainian Academy of Sciences, Ukrainian SSR, Kiev, U.S.S.R.
Neuroscience. 1990;34(3):785-92. doi: 10.1016/0306-4522(90)90183-5.
Cell incubation with lecithin-cholesterol liposomes (1:1 mol/mol) caused enhancement of the cholesterol content. The level of cholesterol esters of total and phospholipid unsaturated fatty acids increased in cholesterol enriched cells. Simultaneously the amount of saturated fatty acids decreased and lysophosphatidylcholine appeared in the cells. On the contrary, cell incubation with lecithin liposomes resulted in cholesterol depletion. This effect was accompanied by a decrease of cholesterol esters, of total and phospholipid unsaturated fatty acids. The content of saturated fatty acids was raised in cells with reduced amount of cholesterol. The quantity of N-acylphosphatidylethanolamine and N-acylethanolamine, lipids newly found in neuroblastoma cells, also changed in cells with modified content of cholesterol. The physiological role of cell response to the changes of cholesterol level is discussed.
用卵磷脂 - 胆固醇脂质体（1:1摩尔/摩尔）孵育细胞会导致胆固醇含量增加。在胆固醇富集的细胞中，总胆固醇酯以及磷脂不饱和脂肪酸的胆固醇酯水平升高。同时，饱和脂肪酸的量减少，细胞中出现溶血磷脂酰胆碱。相反，用卵磷脂脂质体孵育细胞会导致胆固醇耗竭。这种效应伴随着胆固醇酯、总不饱和脂肪酸和磷脂不饱和脂肪酸的减少。在胆固醇含量降低的细胞中，饱和脂肪酸的含量升高。神经母细胞瘤细胞中新发现的脂质N - 酰基磷脂酰乙醇胺和N - 酰基乙醇胺的量在胆固醇含量改变的细胞中也发生了变化。本文讨论了细胞对胆固醇水平变化作出反应的生理作用。
