Gulaia N M, Volkov G L, Govseeva N N, Artemenko I P
Ukr Biokhim Zh (1978). 1986 Jan-Feb;58(1):39-43.
An essential increase in the total cholesterol content with a significant growth in the level of its esters was observed in neuroblastoma C1300 cells differentiated by means of 5'-bromodesoxyuridine and incubated for 1 h with lecithin-cholesterol liposomes (1:1 mol/mol). These cells also displayed an increased amount of polyunsaturated fatty acids in the composition of lecithin molecules and the appearance of lysolicethin as well. Incubation of the cells with lecithin liposomes was accompanied by a decrease in the content of cholincholesterol in the cells. In this case the level of cholesterol esters decreased to a greater extent than the level of free cholesterol. In lecithin molecules of these cells there occurred a relative increase of saturated fatty acids. The cells can retain viability and compensate changes caused by cholesterol excess during their incubation with lecithin-cholesterol liposome for 60-90 min. A longer incubation with liposomes results in a sharp drop of cell viability.
在用5'-溴脱氧尿苷诱导分化并与卵磷脂-胆固醇脂质体（1:1摩尔/摩尔）孵育1小时的神经母细胞瘤C1300细胞中，观察到总胆固醇含量显著增加，其酯水平也显著升高。这些细胞中卵磷脂分子组成中的多不饱和脂肪酸含量也增加，同时出现了溶血卵磷脂。细胞与卵磷脂脂质体孵育后，细胞内胆碱胆固醇含量降低。在这种情况下，胆固醇酯水平的下降幅度大于游离胆固醇水平。这些细胞的卵磷脂分子中饱和脂肪酸相对增加。细胞在与卵磷脂-胆固醇脂质体孵育60 - 90分钟期间能够保持活力并补偿胆固醇过量引起的变化。与脂质体孵育时间延长会导致细胞活力急剧下降。
