Roberson A E, Wolffe A P, Hauser L J, Olins D E
U.T.-Oak Ridge Graduate School of Biomedical Sciences, Biology Division, TN 37831-8077.
Nucleic Acids Res. 1989 Jun 26;17(12):4699-712. doi: 10.1093/nar/17.12.4699.
The macronucleus of the ciliated protozoan Euplotes eurystomus contains about 10(6) copies of a single type of 5S ribosomal RNA gene. This 5S gene DNA is only 930 bp long, is flanked by telomeres, and contains a single coding region of 120 bp which serves as a template for transcription in vivo and in vitro. The 5S gene minichromatin possesses four positioned nucleosomes and hypersensitive cleavage sites in the telomeric regions.
纤毛原生动物阔口游仆虫的大核含有约10⁶个单一类型的5S核糖体RNA基因拷贝。这种5S基因DNA仅930 bp长,两侧为端粒,包含一个120 bp的单一编码区,在体内和体外均作为转录模板。5S基因微染色质在端粒区域具有四个定位核小体和超敏切割位点。