Institute of Neurobiology A. Fessard, Laboratory of Neurobiology and Development, CNRS UPR3294, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, 91198 Gif-sur-Yvette, France.
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2213 Garland Ave, Nashville, TN 37232, USA.
Dev Cell. 2014 Jul 28;30(2):123-36. doi: 10.1016/j.devcel.2014.05.012. Epub 2014 Jul 10.
Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notch invalidations, to address this issue in the zebrafish adult telencephalon. We report that the germinal zone of the adult pallium originates from two distinct subtypes of embryonic progenitors and integrates two modes of aNSC formation. Dorsomedial aNSCs derive from the amplification of actively neurogenic radial glia of the embryonic telencephalon. On the contrary, the lateral aNSC population is formed by stepwise addition at the pallial edge from a discrete neuroepithelial progenitor pool of the posterior telencephalic roof, activated at postembryonic stages and persisting lifelong. This dual origin of the pallial germinal zone allows the temporally organized building of pallial territories as a patchwork of juxtaposed compartments.
关于成年神经干细胞（aNSCs）的胚胎起源和相关异质性知之甚少。我们使用条件性遗传追踪，通过细胞类型特异性启动子或焦点激光光解以全局或镶嵌方式激活，结合基因表达分析和 Notch 无效化，在斑马鱼成年端脑中解决这个问题。我们报告说，成年脑皮层的生发区源自两种不同类型的胚胎祖细胞，并整合了两种 aNSC 形成模式。背内侧 aNSC 来源于胚胎端脑活跃的神经发生性放射状胶质的扩增。相反，侧部 aNSC 群体是由来自后脑顶后离散神经上皮祖细胞池的逐步添加形成的，该祖细胞池在胚胎后阶段被激活并终身持续存在。生发区的这种双重起源允许作为相邻隔室拼贴的脑皮层区域按照时间顺序进行组织构建。
