Packeu A, De Bel A, l'Ollivier C, Ranque S, Detandt M, Hendrickx M
Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium
Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
J Clin Microbiol. 2014 Sep;52(9):3440-3. doi: 10.1128/JCM.01428-14. Epub 2014 Jul 16.
The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.
使用广泛的参考数据库通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)工作流程对皮肤癣菌进行鉴定,在176株临床菌株上进行了评估。培养3天后采用直接沉积法,正确鉴定率为40%。延长培养时间和采用提取法均使正确鉴定率达到100%。