National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, 100193 Beijing, China.
Tianjin Ninghe Primary Pig Breeding Farm, Ninghe 301500, Tianjin, China.
J Anim Sci Biotechnol. 2014 Jun 6;5(1):32. doi: 10.1186/2049-1891-5-32. eCollection 2014.
Fatness traits in animals are important for their growth, meat quality, reproductive performance, and immunity. The liver is the principal organ of the regulation of lipid metabolism, and this study used massive parallelized high-throughput sequencing technologies to determine the porcine liver tissue transcriptome architecture of two full-sibling Songliao black pigs harboring extremely different phenotypes of backfat thickness.
The total number of reads produced for each sample was in the region of 53 million, and 8,226 novel transcripts were detected. Approximately 92 genes were differentially regulated in the liver tissue, while 31 spliced transcripts and 33 primary transcripts showed significantly differential expression between pigs with higher and lower backfat thickness. Genes that were differentially expressed were involved in the metabolism of various substances, small molecule biochemistry, and molecular transport.
Genes involved in the regulation of lipids could play an important role in lipid and fatty acid metabolism in the liver. These results could help us understand how liver metabolism affects the backfat thickness of pigs.
动物的肥胖特征对其生长、肉质、繁殖性能和免疫力都很重要。肝脏是调节脂质代谢的主要器官，本研究使用大规模平行高通量测序技术，确定了两头具有截然不同背脂厚度表型的同父同母的松辽黑猪的猪肝脏组织转录组结构。
每个样本的总读段数在 5300 万左右，检测到 8226 个新的转录本。肝脏组织中约有 92 个基因差异表达，而在背脂厚度较高和较低的猪之间，有 31 个剪接转录本和 33 个初级转录本表现出明显的差异表达。差异表达的基因参与了各种物质的代谢、小分子生物化学和分子运输。
参与脂质调节的基因可能在肝脏的脂质和脂肪酸代谢中发挥重要作用。这些结果有助于我们了解肝脏代谢如何影响猪的背脂厚度。
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