粉红粘帚霉诱导番茄叶片对番茄灰霉病抗性的分析

Analysis of Clonostachys rosea-induced resistance to tomato gray mold disease in tomato leaves.

作者信息

Mouekouba Liana Dalcantara Ongouya, Zhang Lili, Guan Xin, Chen Xiuling, Chen Hongyu, Zhang Jian, Zhang Junfeng, Li Jingfu, Yang Yijun, Wang Aoxue

机构信息

School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, PR China; College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang, PR China; College of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, PR China.

College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang, PR China.

出版信息

PLoS One. 2014 Jul 25;9(7):e102690. doi: 10.1371/journal.pone.0102690. eCollection 2014.

Tomato gray mold disease, caused by Botrytis cinerea, is a serious disease in tomato. Clonostachys rosea is an antagonistic microorganism to B. cinerea. To investigate the induced resistance mechanism of C. rosea, we examined the effects of these microorganisms on tomato leaves, along with changes in the activities of three defense enzymes (PAL, PPO, GST), second messengers (NO, H2O2, O2(-)) and phytohormones (IAA, ABA, GA3, ZT, MeJA, SA and C2H4). Compared to the control, all treatments induced higher levels of PAL, PPO and GST activity in tomato leaves and increased NO, SA and GA3 levels. The expression of WRKY and MAPK, two important transcription factors in plant disease resistance, was upregulated in C. rosea- and C. rosea plus B. cinerea-treated samples. Two-dimensional gel electrophoresis analysis showed that two abundant proteins were present in the C. rosea plus B. cinerea-treated samples but not in the other samples. These proteins were determined (by mass spectrum analysis) to be LEXYL2 (β-xylosidase) and ATP synthase CF1 alpha subunit. Therefore, C. rosea plus B. cinerea treatment induces gray mold resistance in tomato. This study provides a basis for elucidating the mechanism of C. rosea as a biocontrol agent.

由灰葡萄孢引起的番茄灰霉病是番茄的一种严重病害。粉红粘帚霉是灰葡萄孢的一种拮抗微生物。为了研究粉红粘帚霉的诱导抗性机制，我们检测了这些微生物对番茄叶片的影响，以及三种防御酶（苯丙氨酸解氨酶、多酚氧化酶、谷胱甘肽 - S - 转移酶）、第二信使（一氧化氮、过氧化氢、超氧阴离子）和植物激素（生长素、脱落酸、赤霉素、玉米素、茉莉酸甲酯、水杨酸和乙烯）活性的变化。与对照相比，所有处理均诱导番茄叶片中苯丙氨酸解氨酶、多酚氧化酶和谷胱甘肽 - S - 转移酶活性水平升高，并增加了一氧化氮、水杨酸和赤霉素的含量。植物抗病性中两个重要转录因子WRKY和丝裂原活化蛋白激酶（MAPK）的表达在经粉红粘帚霉以及粉红粘帚霉与灰葡萄孢共同处理的样品中上调。二维凝胶电泳分析表明，在粉红粘帚霉与灰葡萄孢共同处理的样品中有两种丰富蛋白存在，而在其他样品中不存在。通过质谱分析确定这些蛋白为LEXYL2（β - 木糖苷酶）和ATP合酶CF1α亚基。因此，粉红粘帚霉与灰葡萄孢共同处理可诱导番茄对灰霉病产生抗性。本研究为阐明粉红粘帚霉作为生防菌的作用机制提供了依据。