Takumida M, Bagger-Sjöbäck D, Rask-Andersen H
Department of Otolaryngology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
ORL J Otorhinolaryngol Relat Spec. 1989;51(5):276-84. doi: 10.1159/000276073.
The effect of acetazolamide on the ultrastructures of the murine endolymphatic sac was investigated. The animals were given a single intravenous dose of acetazolamide (100 mg/b.w.) and were sacrificed 0, 15, 30, 60 and 120 min after the injection, respectively. Prominent changes in the fine structure of the epithelial cells could be observed after 30 min. These alterations were even more pronounced after 1 h. After 2 h, the normal cell structure became to be reestablished. The most conspicuous change was a general reduction in the electron density of the dark cells. This was accompanied with a decreased number of cell organelles, especially ribosomes. Some light cells also underwent temporal modifications in their structure in the form of a reduced nuclear stainability associated with a loss of pinocytotic vesicles near the apical plasmalemma. In general, the dark cells seemed to be more influenced by acetazolamide than the light cells. The possibility that the dark cell changes are related to a modification of transepithelial ion and water flow is discussed.
研究了乙酰唑胺对小鼠内淋巴囊超微结构的影响。给动物单次静脉注射乙酰唑胺（100mg/体重），并分别在注射后0、15、30、60和120分钟处死动物。注射30分钟后可观察到上皮细胞精细结构的显著变化。1小时后这些改变更加明显。2小时后，正常细胞结构开始重新建立。最明显的变化是暗细胞的电子密度普遍降低。这伴随着细胞器数量的减少，尤其是核糖体。一些亮细胞的结构也经历了暂时的改变，表现为核染色性降低，同时顶端质膜附近的胞饮小泡减少。总体而言，暗细胞似乎比亮细胞更容易受到乙酰唑胺的影响。文中讨论了暗细胞变化与跨上皮离子和水流改变相关的可能性。
