Fu Liu-Hui, Hu Kang-Di, Hu Lan-Ying, Li Yan-Hong, Hu Liang-Bin, Yan Hong, Liu Yong-Sheng, Zhang Hua
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
School of Food Science, Henan Institute of Science and Technology, Xinxiang, China.
PLoS One. 2014 Aug 7;9(8):e104206. doi: 10.1371/journal.pone.0104206. eCollection 2014.
In this research, the antifungal role of hydrogen sulfide (H2S) on the postharvest pathogens Aspergillus niger and Penicillium italicum growing on fruits and under culture conditions on defined media was investigated. Our results show that H2S, released by sodium hydrosulfide (NaHS) effectively reduced the postharvest decay of fruits induced by A. niger and P. italicum. Furthermore, H2S inhibited spore germination, germ tube elongation, mycelial growth, and produced abnormal mycelial contractions when the fungi were grown on defined media in Petri plates. Further studies showed that H2S could cause an increase in intracellular reactive oxygen species (ROS) in A. niger. In accordance with this observation we show that enzyme activities and the expression of superoxide dismutase (SOD) and catalase (CAT) genes in A. niger treated with H2S were lower than those in control. Moreover, H2S also significantly inhibited the growth of Saccharomyces cerevisiae, Rhizopus oryzae, the human pathogen Candida albicans, and several food-borne bacteria. We also found that short time exposure of H2S showed a microbicidal role rather than just inhibiting the growth of microbes. Taken together, this study suggests the potential value of H2S in reducing postharvest loss and food spoilage caused by microbe propagation.
在本研究中，研究了硫化氢（H₂S）对采后病原菌黑曲霉和意大利青霉在水果上以及在特定培养基培养条件下生长的抗真菌作用。我们的结果表明，由硫氢化钠（NaHS）释放的H₂S有效降低了黑曲霉和意大利青霉引起的水果采后腐烂。此外，当真菌在培养皿中的特定培养基上生长时，H₂S抑制孢子萌发、芽管伸长、菌丝生长，并产生异常的菌丝收缩。进一步的研究表明，H₂S可导致黑曲霉细胞内活性氧（ROS）增加。根据这一观察结果，我们发现用H₂S处理的黑曲霉中，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）基因的酶活性及表达低于对照。此外，H₂S还显著抑制酿酒酵母、米根霉、人类病原菌白色念珠菌以及几种食源细菌的生长。我们还发现，短时间暴露于H₂S显示出杀菌作用，而不仅仅是抑制微生物生长。综上所述，本研究表明H₂S在减少采后损失和微生物繁殖引起的食品腐败方面具有潜在价值。
