Barde P V, Shukla M K, Pathak Ruchi, Kori B K, Bharti P K
Regional Medical Research Centre for Tribals (ICMR), Jabalpur, India.
Indian J Med Res. 2014 Jun;139(6):940-4.
Hepatitis A virus (HAV) infection, a major cause of childhood hepatitis is transmitted by orofaecal route. Children mostly suffer with subclinical infection but may have serious clinical implications leading to hospitalization and mortality. IgM ELISA and nRT PCR were conducted on the blood samples collected from HAV suspected paediatric cases referred to the viral diagnostic laboratory in the Regional Medical Research Centre for Tribals at Jabalpur, Central India. The nRT PCR products were sequenced and phylogenetic analysis was done. Of the 195 samples tested, 41 (21%) were positive for HAV antibodies, among which 38 (92%) belonged to paediatric age group and 32 per cent of these were hospitalized. nRT PCR and sequencing confirmed the presence of HAV. Phylogenic analysis revealed circulation of genotype III A in central India. Regular serological and molecular monitoring would aid in understanding epidemiology of HAV and plan intervention strategies.
甲型肝炎病毒（HAV）感染是儿童肝炎的主要病因，通过粪口途径传播。儿童大多遭受亚临床感染，但可能会产生严重的临床后果，导致住院和死亡。对从印度中部贾巴尔普尔部落地区医学研究中心的病毒诊断实验室转诊的疑似HAV儿科病例采集的血样进行了IgM ELISA和逆转录巢式聚合酶链反应（nRT PCR）检测。对nRT PCR产物进行测序并进行系统发育分析。在检测的195份样本中，41份（21%）HAV抗体呈阳性，其中38份（92%）属于儿童年龄组，这些儿童中有32%住院治疗。nRT PCR和测序证实了HAV的存在。系统发育分析显示印度中部存在III A基因型的传播。定期的血清学和分子监测将有助于了解HAV的流行病学情况并制定干预策略。
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