Ameen Alyusif, Sabeena Sasidharanpillai, Robin Sudandiradas, Sanjay Ramachandran, Prasad Varamballi, Mevis Fernandes M, Bhatt Puneet, Arunkumar Govindakarnavar
Research Assistant (Clinical Virology), Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.
Assistant Professor, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.
Med J Armed Forces India. 2022 Apr;78(2):185-191. doi: 10.1016/j.mjafi.2020.11.019. Epub 2021 Feb 26.
This study was carried out to understand the circulating genotypes of Hepatitis A virus (HAV) in South West, East and North East India during the period 2017-2018 as a part of acute febrile illness surveillance at the Manipal Institute of Virology.
Archived serum samples of 48 Hepatitis A confirmed cases were subjected to RNA extraction using QIAamp® Viral RNA Mini Kit (QIAGEN, Germany). The samples with molecular confirmation for HAV by reverse transcriptase real-Time PCR (Real Star® HAV RT-PCR Kit 2.0, Altona Diagnostics, GmbH, Hamburg, Germany) were further subjected to nested conventional PCR targeting the 5' UTR region. The purified PCR products were sequenced using Big Dye Terminator Kit (Applied Biosystems, USA), in a 3500 XL genetic analyzer (Applied Biosystems, USA). The edited sequences by means of MEGA X (MEGA version 10.1) were compared with reference sequences in the NCBI nucleotide database.
From states of Assam, Goa, Gujarat, Karnataka, Kerala, Maharashtra, Odisha, Tamil Nadu and Tripura, 139 Hepatitis A and 33 Hepatitis E cases were reported during the study period. The median age of the acute Hepatitis A cases was 19 years (IQR 12.8-24) and most of the affected individuals were students between 10 and 19 years (52.5%). In the present study, 14 samples from Assam, Goa, Gujarat, Karnataka, Odisha, Kerala, Maharashtra and Tamil Nadu were genotyped as genotype IIIA by nested conventional polymerase chain reaction.
The circulating HAV genotype in South West, North East and East India between 2017 and 2018 was IIIA.
作为马尼帕尔病毒学研究所急性发热性疾病监测的一部分，本研究旨在了解2017 - 2018年期间印度西南部、东部和东北部甲型肝炎病毒（HAV）的流行基因型。
使用QIAamp®病毒RNA迷你试剂盒（德国QIAGEN公司）对48例确诊甲型肝炎病例的存档血清样本进行RNA提取。通过逆转录实时PCR（Real Star® HAV RT-PCR试剂盒2.0，德国汉堡Altona Diagnostics GmbH公司）对HAV进行分子确认的样本，进一步进行针对5' UTR区域的巢式常规PCR。纯化的PCR产物使用Big Dye Terminator试剂盒（美国应用生物系统公司）在3500 XL基因分析仪（美国应用生物系统公司）中进行测序。通过MEGA X（MEGA版本10.1）编辑的序列与NCBI核苷酸数据库中的参考序列进行比较。
在研究期间，从阿萨姆邦、果阿邦、古吉拉特邦、卡纳塔克邦、喀拉拉邦、马哈拉施特拉邦、奥里萨邦、泰米尔纳德邦和特里普拉邦报告了139例甲型肝炎病例和33例戊型肝炎病例。急性甲型肝炎病例的中位年龄为19岁（四分位间距12.8 - 24），大多数受影响个体为10至19岁的学生（52.5%）。在本研究中，来自阿萨姆邦、果阿邦、古吉拉特邦、卡纳塔克邦、奥里萨邦、喀拉拉邦、马哈拉施特拉邦和泰米尔纳德邦的14个样本通过巢式常规聚合酶链反应被基因分型为IIIA型。
2017年至2018年期间，印度西南部、东北部和东部流行的HAV基因型为IIIA型。
