印度北方邦甲型肝炎病毒流行株的分子特征：一项基于医院的研究。

Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study.

机构信息

Department of Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, India.

出版信息

Indian J Med Res. 2020 Apr;151(4):375-379. doi: 10.4103/ijmr.IJMR_429_18.

DOI:10.4103/ijmr.IJMR_429_18

PMID:32461402

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7371058/

Abstract

BACKGROUND & OBJECTIVES: Hepatitis A is prevalent worldwide and is among the leading cause of acute viral hepatitis in India. Major geographical differences in endemicity of hepatitis A are closely related to hygienic and sanitary conditions and other indicators of the level of socio-economic development. The present study was aimed to know the seropositivity prevalence and predominant circulating strain of HAV in a north India.

METHODS

Patients with acute viral hepatitis were enrolled. Blood samples were collected over a period of one year from June 2016 to May 2017. Serum samples were tested for anti-immunoglobulin M (IgM) HAV antibodies. The seropositive samples were analyzed for HAV-RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Samples detected on molecular assay were subjected to conventional semi-nested RT-PCR for VP1 gene. Further sequencing of amplified RT-PCR products was done, and data were analyzed.

RESULTS

A total of 1615 patients were enrolled, and serum samples were collected and tested. The male:female ratio was 1.3:1 with a mean age of 24.31±17.02 yr (range 0-83 yr). Among these, 128 (7.93%) were positive for anti-HAV IgM antibodies; 41.63 per cent of seropositive patients were in their childhood or early adolescent age group. Of all seropositive samples, 59 (46.09%) were positive for HAV RNA. Genotyping sequencing of 10 representative strains was carried out, and the circulating genotype was found to be IIIA. The nucleotide sequences showed homology among the strains.

INTERPRETATION & CONCLUSIONS: Our results showed that hepatitis A was a common disease in children with IIIA as a circulating genotype in this region. In approximately 50 per cent of cases, HAV RNA could be detected. Higher number of HAV IgM-seropositive cases was observed during monsoon period.

摘要

背景与目的

甲型肝炎在全球广泛流行，是印度急性病毒性肝炎的主要病因之一。甲型肝炎的地方性流行在很大程度上与卫生和卫生条件以及其他社会经济发展水平指标有关。本研究旨在了解印度北部甲型肝炎的血清阳性率流行情况和主要流行株。

方法

纳入急性病毒性肝炎患者。从 2016 年 6 月至 2017 年 5 月，在一年期间采集血液样本。检测血清样本中抗免疫球蛋白 M（IgM）甲型肝炎病毒抗体。对血清阳性样本进行实时逆转录聚合酶链反应（RT-PCR）检测甲型肝炎病毒 RNA。对分子检测阳性的样本进行常规半巢式 RT-PCR 检测 VP1 基因。对扩增的 RT-PCR 产物进行测序，并进行数据分析。

结果

共纳入 1615 例患者，采集并检测血清样本。男女比例为 1.3:1，平均年龄为 24.31±17.02 岁（0-83 岁）。其中，128 例（7.93%）抗-HAV IgM 抗体阳性；41.63%的血清阳性患者处于儿童或青少年早期年龄组。在所有血清阳性样本中，59 例（46.09%）甲型肝炎病毒 RNA 阳性。对 10 个代表性毒株进行基因分型测序，发现流行株为 IIIA 型。株间核苷酸序列同源。