Burdon R H, Gill V, Rice-Evans C
Department of Bioscience and Biotechnology, Todd Centre, University of Strathclyde, Glasgow, UK.
Free Radic Res Commun. 1989;7(3-6):149-59. doi: 10.3109/10715768909087937.
Antioxidants such as mannitol, butylated hydroxytoluene and alpha-tocopherol enhance the growth of polyoma virus transformed and non-transformed BHK-21 cells. In the case of mannitol this is observed even in the absence of added calf serum. In part these effects may operate to protect cellular growth control mechanisms. On the other hand oxidants such as H2O2 and t-butyl hydroperoxide can inhibit growth and overall cellular protein synthesis, through mechanisms that are likely to involve radicals. In the case of H2O2 the inhibitory effects can nevertheless be reduced by 'prestressing' the cells with mild heat or with H2O2 itself. Paradoxically very low concentrations (10(-8) M) of H2O2 or t-butyl hydroperoxide can actually stimulate cell growth, even in the absence of serum. These stimulatory effects however do not appear to involve radicals as they are enhanced by inclusion of mannitol or DMSO in the medium.
抗氧化剂如甘露醇、丁基化羟基甲苯和α-生育酚可促进多瘤病毒转化和未转化的BHK - 21细胞的生长。就甘露醇而言，即使在不添加小牛血清的情况下也能观察到这种促进作用。这些作用部分可能是通过保护细胞生长控制机制来实现的。另一方面，氧化剂如过氧化氢和叔丁基过氧化氢可抑制细胞生长和整体细胞蛋白质合成，其机制可能涉及自由基。就过氧化氢而言，通过用温和的热或过氧化氢本身对细胞进行“预应激”，其抑制作用可被降低。矛盾的是，即使在无血清的情况下，极低浓度（10⁻⁸ M）的过氧化氢或叔丁基过氧化氢实际上也能刺激细胞生长。然而，这些刺激作用似乎不涉及自由基，因为在培养基中加入甘露醇或二甲基亚砜可增强这些作用。
