Lili Geng, Deng Xu, Minhong Zhang, Changlong Shu, Jinghai Feng, Fuping Song, Fan Lu, Jie Zhang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Microalgae Laboratory, School of Resource & Environmental Engineering, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
J Poult Sci. 2018;55(1):10-16. doi: 10.2141/jpsa.0170029. Epub 2017 Aug 25.
Many types of (Bt)-crops are being grown worldwide, triggering concerns about their potential impact on humans and livestock. To ensure better yield and food safety in China, an attempt has been made to develop Bt-rice targeting a broad range of insects. We aimed to investigate whether feeding genetically modified rice expressing the Bt chimeric Cry1Ac/Cry1Ab protein has any effects on the intestinal microbiota of broilers. Broilers were fed either Bt-rice or its unmodified isogenic parent line for 42 days, and total DNA was isolated from cecum contents for high-throughput sequencing of the 16S rRNA gene. In total, 1,241,005 reads, assigned to 12 phyla, 31 families, and 48 genera were generated. No significant differences were observed in the relative abundance of organisms identified among the major phyla, families, and genera, except for two less abundant families, and , and two less abundant genera, and . The results were in agreement with those from culture-based analysis and Biolog EcoPlates. These results illustrate that feeding Bt-rice has no adverse effects on the broiler intestinal microbiota and provide sufficient support for the food safety of Bt-rice.
全球正在种植多种类型的转基因抗虫（Bt）作物，这引发了人们对其对人类和牲畜潜在影响的担忧。为确保中国有更高的产量和食品安全，人们已尝试培育针对多种昆虫的转基因抗虫水稻。我们旨在研究喂食表达Bt嵌合Cry1Ac/Cry1Ab蛋白的转基因水稻是否会对肉鸡的肠道微生物群产生任何影响。将肉鸡喂食转基因抗虫水稻或其未转基因的同基因亲本系42天，从盲肠内容物中分离总DNA用于16S rRNA基因的高通量测序。总共产生了1,241,005条读数，归属于12个门、31个科和48个属。在主要的门、科和属中鉴定出的生物相对丰度没有观察到显著差异，除了两个丰度较低的科，即 和 ，以及两个丰度较低的属，即 和 。结果与基于培养的分析和Biolog生态板的结果一致。这些结果表明，喂食转基因抗虫水稻对肉鸡肠道微生物群没有不利影响，并为转基因抗虫水稻的食品安全提供了充分支持。
