秘鲁成年人白细胞介素-10基因-1082位点多态性与慢性牙周炎的关联
Association of -1082 interleukin-10 gene polymorphism in Peruvian adults with chronic periodontitis.
作者信息
Chambrone Leandro, Ascarza Amilcar, Guerrero Maria-Eugenia, Pannuti Claudio, de la Rosa Manuel, Salinas-Prieto Elmer, Mendoza Gerardo
机构信息
Department of Periodontology - School of Dentistry, Científica del Sur University, Av. Paseo de la República N 5544, Lima 18 Peru,
出版信息
Med Oral Patol Oral Cir Bucal. 2014 Nov 1;19(6):e569-73. doi: 10.4317/medoral.19823.
OBJECTIVES
The aim of this study was to assess association of the -1082 IL-10 gene polymorphism with chronic periodontitis CP in a Peruvian population.
STUDY DESIGN
Samples of venous blood and DNA were obtained from 106 Peruvian subjects: a) 53 periodontally healthy; and b) 53 with CP. The association of the -1082 IL-10 promoter sequences was assessed by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Student's t test were used to assess the clinical parameters, as well as the χ2 test and the odds ratio (OR), with 95% confidence intervals (CI) used performed for estimates regarding genotype and allele frequencies.
RESULTS
There were statistically significant differences between groups regarding the mean bleeding on probing, mean attachment level and mean probing depth (p < 0.00001) indicating that the matching based on the evaluated groups was adequate. The χ2 test found a statistically significant imbalance of genotypes between groups (p = 0.0172). The prevalence of CP was significantly higher in subjects harboring at least one A allele at position -1082 (AA and GA genotypes) in comparison to patients with the GG genotype (OR = 2.96; CI: 0.52; 5.41; p = 0.0099). Equally, subjects with the AA genotype were significantly associated to a diagnosis of CP (OR = 2.71; CI: 0.38; 5.04; p = 0.0231). On the other hand, subjects presenting a healthy periodontal status presented at least one G allele in comparison with the AA genotype (OR = 0.37; CI: 0.05, 0.69; p = 0.0231). For subjects with the GG genotype, the same positive association was observed (OR = 0.34; CI: 0.06, 0.62; p = 0.0099). There were no significant differences between groups amongst subjects with the GA genotype (OR = 1.19; CI: 0.22, 2.16; p = 0.6774).
CONCLUSIONS
Within the limits of this study, IL-10 gene polymorphism at position -1082 does not appear to be associated to CP. Conversely, subjects with AA genotype seem to be at an increased risk of developing CP.
目的
本研究旨在评估秘鲁人群中白细胞介素-10（IL-10）基因-1082位点多态性与慢性牙周炎（CP）的相关性。
研究设计
从106名秘鲁受试者中采集静脉血和DNA样本：a）53名牙周健康者；b）53名患有CP者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估-1082 IL-10启动子序列的相关性。采用学生t检验评估临床参数，以及χ2检验和比值比（OR），并使用95%置信区间（CI）对基因型和等位基因频率进行估计。
结果
两组在探诊出血均值、附着丧失均值和探诊深度均值方面存在统计学显著差异（p < 0.00001），表明基于评估组的匹配是合适的。χ2检验发现两组间基因型存在统计学显著失衡（p = 0.0172）。与GG基因型患者相比，在-1082位点至少携带一个A等位基因（AA和GA基因型）的受试者中CP患病率显著更高（OR = 2.96；CI：0.52；5.41；p = 0.0099）。同样，AA基因型受试者与CP诊断显著相关（OR = 2.71；CI：0.38；5.04；p = 0.0231）。另一方面，与AA基因型相比，牙周健康状态的受试者至少携带一个G等位基因（OR = 0.37；CI：0.05，0.69；p = 0.0231）。对于GG基因型受试者，观察到相同的正相关（OR = 0.34；CI：0.06，0.62；p = 0.0099）。GA基因型受试者组间无显著差异（OR = 1.19；CI：0.22，2.16；p = 0.6774）。
结论
在本研究范围内，-1082位点的IL-10基因多态性似乎与CP无关。相反，AA基因型受试者似乎患CP的风险增加。
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