Aggarwal R, Gupta S, Nijhawan R, Suri V, Kaur A, Bhasin V, Arora S K
Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
Indian J Cancer. 2006 Jul-Sep;43(3):110-6. doi: 10.4103/0019-509x.27932.
Cervical cancer is the commonest cancer among Indian women. High-risk human papillomavirus (HPV) detection holds the potential to be used as a tool to identify women, at risk for subsequent development of cervical cancer. There is a pressing need for identifying prevalence of asymptomatic cervical HPV infection in the local population.
To determine the prevalence of high-risk HPV DNA in women with benign cervical cytology.
Women visiting the gynecology outpatient with varied complaints were subjected to Pap smear. Four hundred and seventy two samples were subjected to polymerase chain reaction, using consensus primers for low and high-risk HPV (types 6, 11, 16, 18, 31 and 33). The samples that were positive for HPV DNA were subsequently assessed for high-risk consensus primers, types 16, 18, 31 and 33 as well as for HPV type 16 and 18.
One hundred and seventy four (36.8%) women tested positive for HPV DNA. Thirty nine (8.2%) of the entire cohort tested positive for high-risk HPV. Fifteen samples were positive for type 16, 22 for type 18 and two for both types 16 and 18. A statistically higher prevalence of high-risk HPV was observed in poorly educated and rural groups. No association of HPV prevalence was noted with age, parity and age at marriage.
The study generates epidemiological data of prevalence of sub-clinical HPV in the women visiting a tertiary care institute as well as peripheral health centres. The data generated will be useful for laying guidelines for mass screening of HPV, treatment and prophylaxis in the local population.
宫颈癌是印度女性中最常见的癌症。高危型人乳头瘤病毒（HPV）检测有望成为识别有患宫颈癌风险女性的一种工具。迫切需要确定当地人群中无症状宫颈HPV感染的患病率。
确定宫颈细胞学检查为良性的女性中高危型HPV DNA的患病率。
对因各种妇科问题前来门诊的女性进行巴氏涂片检查。472份样本采用针对低危和高危型HPV（6、11、16、18、31和33型）的通用引物进行聚合酶链反应。对HPV DNA检测呈阳性的样本，随后用高危通用引物（16、18、31和33型）以及HPV 16和18型进行评估。
174名（36.8%）女性HPV DNA检测呈阳性。整个队列中有39名（8.2%）女性高危型HPV检测呈阳性。15份样本16型呈阳性，22份18型呈阳性，2份16和18型均呈阳性。在受教育程度低的人群和农村人群中，观察到高危型HPV的患病率在统计学上更高。未发现HPV患病率与年龄、产次和结婚年龄有关。
本研究得出了在三级医疗机构和周边健康中心就诊女性中亚临床HPV患病率的流行病学数据。所生成的数据将有助于为当地人群的HPV大规模筛查、治疗和预防制定指导方针。
