Martens Michiel A, Boesmans Werend, Vanden Berghe Pieter
Laboratory for Enteric Neuroscience (LENS),TARGID, University of Leuven, O&N 1 Herestraat 49-box 701, Leuven 3000, Belgium.
Biomed Opt Express. 2014 Jul 16;5(8):2648-61. doi: 10.1364/BOE.5.002648. eCollection 2014 Aug 1.
We have configured a widefield fast imaging system that allows imaging at 1000 frames per second (512x512 pixels). The system was extended with custom processing tools including a time correlation method to facilitate the analysis of static subcellular compartments (e.g. neuronal varicosities) with enhanced contrast, as well as a dynamic intensity processing (DIP) algorithm that aids in data size reduction and fast visualization and interpretation of timing and directionality in neuronal circuits. This system, together with our custom developed processing tools enables efficient detection of fast physiological events, such as action potential dependent calcium steps. We show, using a specific blocker of nerve communication, that with this setup it is possible to discriminate between a pre and post synaptic event in an all optical way.
我们配置了一个宽场快速成像系统,该系统能够以每秒1000帧(512x512像素)的速度进行成像。该系统通过定制处理工具进行了扩展,包括一种时间相关方法,以促进对静态亚细胞区室(如神经元曲张体)进行对比度增强的分析,以及一种动态强度处理(DIP)算法,该算法有助于减小数据大小,并能快速可视化和解释神经元回路中的时间和方向性。该系统与我们定制开发的处理工具一起,能够高效检测快速生理事件,如动作电位依赖性钙瞬变。我们使用一种神经通讯的特异性阻断剂表明,通过这种设置,可以以全光学方式区分突触前和突触后事件。