巴西中西部马托格罗索州登革热疫情期间马亚罗病毒的分子检测
Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil.
作者信息
Zuchi Nayara, Heinen Letícia Borges da Silva, Santos Marcelo Adriano Mendes dos, Pereira Fernanda Carla, Slhessarenko Renata Dezengrini
机构信息
Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
MT Laboratório, Secretaria Estadual de Saúde, Cuiabá, MT, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2014 Sep;109(6):820-3. doi: 10.1590/0074-0276140108. Epub 2014 Aug 19.
Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. MAYV was detected in 15 (2.5%) of 604 patients. Twelve were co-infected with dengue virus 4, which was isolated from 10 patients. The molecular detection of MAYV in dengue-suspected patients suggests that other arboviruses may be silently circulating during dengue outbreaks in Brazil.
马亚罗病毒（MAYV）在泛亚马逊地区屡有报道。本研究旨在调查巴西马托格罗索州登革热疫情期间甲病毒的传播情况。对疑似登革热患者的血清样本进行了针对11种黄病毒和5种甲病毒的多重半巢式逆转录聚合酶链反应、核苷酸测序及病毒分离。在604名患者中的15名（2.5%）检测到了马亚罗病毒。其中12名患者同时感染了登革病毒4型，从10名患者中分离出了该病毒。在疑似登革热患者中对马亚罗病毒进行分子检测表明，在巴西登革热疫情期间可能有其他虫媒病毒在悄然传播。
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