Ritland C, Straus N A
Department of Botany, University of Toronto, Ontario, Canada.
Plant Mol Biol. 1993 Jul;22(4):691-6. doi: 10.1007/BF00047409.
Ribosomal DNA sequences for the ITS 1, 5.8S, ITS 2 and adjoining regions of the 18S and 25S were obtained from Mimulus glaucescens (Scrophulariaceae) via cloned PCR products. The spacer sequences were completely unrelated to other plant taxa, although spacer lengths were approximately the same. Interestingly, the Mimulus 5.8S sequence was much more divergent than other higher-plant rDNA sequences. Consideration of the secondary structure of the 5.8S rRNA shows that most of the changes in Mimulus are compensatory and preserve the basic secondary structure of the mature RNA molecule.
通过克隆的聚合酶链式反应（PCR）产物，从沟酸浆（玄参科）中获得了核糖体DNA的ITS 1、5.8S、ITS 2以及18S和25S相邻区域的序列。间隔序列与其他植物类群完全不相关，尽管间隔长度大致相同。有趣的是，沟酸浆的5.8S序列比其他高等植物的核糖体DNA序列差异大得多。对5.8S核糖体RNA二级结构的研究表明，沟酸浆中的大多数变化是补偿性的，并保留了成熟RNA分子的基本二级结构。
