Karoune Samira, Falleh Hanen, Kechebar Mohamed Seif Allah, Halis Youcef, Mkadmini Khaoula, Belhamra Mohamed, Rahmoune Chaabane, Ksouri Riadh
a Laboratoire d'Ecotoxicologie et stress abiotiques, Dépt Biologie et Ecologie , Faculté SNV, Université Constantine 1 , Constantine , Algeria.
Nat Prod Res. 2015;29(5):452-4. doi: 10.1080/14786419.2014.947497. Epub 2014 Aug 21.
This study compared phenolic contents and antioxidant activity in different organs of Acacia albida (leaves and bark) and focuses on identification of phenolic compounds of leaves by HPLC-DAD. The analysed organs exhibited differences in total polyphenol contents (100 and 59.5 mg GAE g(-1) DW). Phenolic contents of leaves were two times higher than those in bark. Ethanolic extracts exhibited good antioxidant activities with IC50 = 26 μg mL(-1) for DPPH and EC50 = 50 μg mL(-1) for FRAP. Identification by HPLC-DAD revealed the presence of nine phenolic compounds known for their high antioxidant activity. The results suggested that this species can be used as source of natural antioxidants.
本研究比较了阿拉伯胶树不同器官(树叶和树皮)中的酚类含量及抗氧化活性,并着重通过高效液相色谱-二极管阵列检测法(HPLC-DAD)鉴定树叶中的酚类化合物。所分析的器官在总多酚含量上存在差异(分别为100和59.5毫克没食子酸当量/克干重)。树叶中的酚类含量是树皮中的两倍。乙醇提取物表现出良好的抗氧化活性,对二苯基苦味酰基自由基(DPPH)的半数抑制浓度(IC50)为26微克/毫升,对铁还原抗氧化能力(FRAP)的半数有效浓度(EC50)为50微克/毫升。通过HPLC-DAD鉴定发现存在九种以高抗氧化活性著称的酚类化合物。结果表明该物种可作为天然抗氧化剂的来源。