Environ Technol. 2014 Sep-Oct;35(17-20):2476-82. doi: 10.1080/09593330.2014.911356.
This study investigates the effect of applying a mixture of enzymes (ME) to dairy cattle manure (DCM) as substrate in anaerobic digestion (AD). The aims of this study were to evaluate different methods of ME application to DCM at different temperatures and to investigate the effect of adding ME during the pre-treatment of the solid fractions of dairy cattle manure (SFDCM). The results showed that there was no positive effect of direct ME addition to substrate at either mesophilic (35 degrees C) or thermophilic (50 degrees C) process temperatures, but there was a significant 4.44% increase in methane yield when DCM, which had been incubated with ME addition at 50 degrees C for three days, was fed to a digester when compared to a control digester operating at the same retention time. Methane production was detected during the pre-treatment incubation, and the total sum methane yield during pre-treatment and digestion was found to be 8.33% higher than in the control. The addition of ME to the SFDCM in a pre-incubation stage of 20 h at 35 degrees C gave a significant increase in methane yield by 4.15% in a digester treating a mixed substrate (30% liquid fractions DCM and 70% enzyme-treated SFDCM) when compared with the control digester treating a similar mixed substrate with inactivated enzyme addition. The results indicate that direct physical contact of enzyme molecules and organic material in DCM prior to AD, without the intervention of extracellular enzymes from the indigenous microorganism population, was needed in order to increase methane yields.
本研究调查了在厌氧消化（AD）中应用混合酶（ME）作为底物对奶牛粪便（DCM）的影响。本研究的目的是评估在不同温度下将 ME 以不同方式应用于 DCM，以及研究在预处理奶牛粪便的固体部分（SFDCM）时添加 ME 的效果。结果表明，在中温（35°C）或高温（50°C）工艺温度下，直接将 ME 添加到基质中均没有产生积极效果，但当与在相同停留时间下运行的对照消化器相比，将在 50°C 下孵育三天后添加 ME 的 DCM 进料到消化器时，甲烷产量增加了 4.44%。在预处理孵育过程中检测到甲烷产生，并且发现预处理和消化过程中的总甲烷产量比对照高 8.33%。在 35°C 下进行 20 小时的预孵育阶段将 ME 添加到 SFDCM 中，与对照消化器处理类似的混合底物（30%液体 DCM 分数和 70%酶处理 SFDCM）相比，处理混合底物（30%液体 DCM 分数和 70%酶处理 SFDCM）的消化器中甲烷产量显著增加了 4.15%。结果表明，在 AD 之前，需要 ME 分子与 DCM 中的有机物质直接物理接触，而无需来自土著微生物种群的细胞外酶的干预，才能提高甲烷产量。
