Mier-Giraldo Helen, Díaz-Barrera Luis Eduardo, Delgado-Murcia Lucy Gabriela, Valero-Valdivieso Manuel Fernando, Cáez-Ramírez Gabriela
1 Universidad de La Sabana, Campus Puente del Común, Cundinamarca, Colombia.
2 Universidad Nacional de Colombia, Bogota, Colombia.
J Evid Based Complementary Altern Med. 2017 Oct;22(4):777-787. doi: 10.1177/2156587217718751. Epub 2017 Jul 18.
It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.
目的是评估成熟灯笼果果实乙醇和异丙醇粗提物的生物学潜力。在人宫颈癌（HeLa）细胞和鼠成纤维细胞（L929）上评估白细胞介素-6、白细胞介素-8和单核细胞趋化蛋白-1（MCP-1）表达的细胞毒性和免疫调节作用。通过高效液相色谱二极管阵列检测和高效液相色谱紫外/可见光检测对成分进行评估。在两种溶剂中均发现了熊果酸和迷迭香酸。然而，异丙醇提取物中的没食子酸、槲皮素和表儿茶素含量更高（P <.05）。结果表明总多酚含量、抗氧化活性和细胞毒性活性之间存在依赖于所用溶剂的关系。异丙醇提取物对HeLa细胞的半数抑制浓度值（IC）为60.48±3.8μg/mL，对L929成纤维细胞为66.62±2.67μg/mL。提取物以剂量依赖的方式减少白细胞介素-6、白细胞介素-8和MCP-1的释放。提取物显示出用于开发新型补充药物产品的抗癌和免疫调节潜力。
