Kachkoul Rabie, Houssaini Tarik Squalli, Mohim Mohamed, El Habbani Radouane, Lahrichi Anissa
Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco.
Faculty of Science and Technology, Sidi Mohammed Ben Abdellah University, BP 2202, Road of Imouzzer, Fez, Morocco.
Evid Based Complement Alternat Med. 2020 Feb 24;2020:9424510. doi: 10.1155/2020/9424510. eCollection 2020.
The plant L. has several biological activities and a great curative and preventive power against chronic diseases. For this purpose, the objective of this work is to valorize the fruit peel of this plant in the field of phytomedicine, by quantifying and identifying its bioactive compounds and by evaluating their antioxidant and anticrystallization activities against calcium oxalate. This comparative study has been carried out by hydroalcoholic extract (E.PG) and infusion (I.PG) of the plant. The quantification of the phenolic compounds has been performed by spectrophotometric methods, and the chemical species identification has been performed by UPLC-PDA-ESI-MS. Moreover, the examination of the antioxidant activity has been executed by both methods of DPPH and FRAP. The crystallization inhibition has been studied in vitro by the turbidimetric model. The characterization of the synthesized crystals has been accomplished by microscopic observation and by Fourier Transform Infrared Spectroscopy. The results found show the comparable importance of the two plant extracts in the elimination of free radicals; the values of the half maximal inhibitory concentration "IC" obtained are in the order of 60.87 ± 0.27 and 59.91 ± 0.83 g/mL by the DPPH method and in the order of 42.17 ± 7.46 and 79.77 ± 6.91 g/mL by the FRAP method, for both E.PG and I.PG, respectively. Furthermore, the inhibition percentages of calcium oxalate crystallization are in the range of 98.11 ± 0.17 and 98.22 ± 0.71% against the nucleation and in the order of 88.98 ± 0.98 and 88.78 ± 2.48% against the aggregation, for E.PG and I.PG, respectively. These results prove the richness of the plant in bioactive compounds, offering an antioxidant and anticrystallization capacity; therefore, it can be used in the treatment and/or the prevention of stone formation.
植物L.具有多种生物活性，对慢性疾病具有强大的治疗和预防作用。为此，本研究的目的是通过对该植物果皮中的生物活性化合物进行定量和鉴定，并评估其对草酸钙的抗氧化和抗结晶活性，从而在植物医学领域对该植物果皮进行价值评估。本对比研究采用了该植物的水醇提取物（E.PG）和浸剂（I.PG）。酚类化合物的定量采用分光光度法，化学物质鉴定采用超高效液相色谱-光电二极管阵列-电喷雾电离质谱法。此外，抗氧化活性检测采用了二苯基苦味酰基自由基（DPPH）法和铁离子还原抗氧化能力（FRAP）法。通过比浊法模型在体外研究了结晶抑制情况。合成晶体的表征通过显微镜观察和傅里叶变换红外光谱法完成。研究结果表明，两种植物提取物在清除自由基方面具有相当的重要性；通过DPPH法获得的半数抑制浓度“IC”值，E.PG和I.PG分别为60.87±0.27和59.91±0.83 g/mL，通过FRAP法获得的值分别为42.17±7.46和79.77±6.91 g/mL。此外，E.PG和I.PG对草酸钙结晶的抑制率分别为98.11±0.17%和98.22±0.71%（针对成核），以及88.98±
