Liu Rui, Zheng Wenwen, Li Jun, Wang Lingchong, Wu Hao, Wang Xinzhi, Shi Lei
Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing 210023, PR China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
Yuhuangding Hospital, Yantai 264000, PR China.
Food Chem. 2015 Jan 15;167:484-9. doi: 10.1016/j.foodchem.2014.06.113. Epub 2014 Jul 12.
Analysis of peptide components of protein hydrolysates is often difficult due to the lack of suitable analytical methods. In the present study, an UHPLC-Q-TOF MS/MS method was developed and used to identify peptides derived from the protein hydrolysate of Mactra veneriformis. The peptide sequences were deduced by de novo sequencing based on MS/MS fragmentation data. A total of 21 peptides, four nucleobases, and one nucleoside were identified from the hydrolysate using this method. These peptides were chemically synthesised and showed antioxidant activity in radical scavenging assays. This method is suitable for quick, sensitive, and accurate analysis of complex protein hydrolysates.
由于缺乏合适的分析方法，对蛋白质水解产物的肽成分进行分析往往很困难。在本研究中，开发了一种超高效液相色谱-四极杆-飞行时间串联质谱（UHPLC-Q-TOF MS/MS）方法，并用于鉴定文蛤蛋白质水解产物中的肽。基于串联质谱碎片数据通过从头测序推导肽序列。使用该方法从水解产物中总共鉴定出21种肽、四种核碱基和一种核苷。这些肽经过化学合成，并在自由基清除试验中显示出抗氧化活性。该方法适用于对复杂蛋白质水解产物进行快速、灵敏和准确的分析。
