Weiss L, Haeffner-Cavaillon N, Laude M, Gilquin J, Kazatchkine M D
INSERM U28, Hôpital Broussais, Paris, France.
AIDS. 1989 Nov;3(11):695-9. doi: 10.1097/00002030-198911000-00002.
Interleukin-1 (IL-1) is not constitutively produced by normal human monocytes. We have investigated the production of cell-associated IL-1 in uncultured unstimulated adherent monocytes from HIV-infected patients, which reflects ongoing generation of IL-1 by the cells in vivo. High levels of cell-associated IL-1 activity and of cell-associated IL-1 alpha and IL-1 beta antigens were found in monocytes from HIV-infected patients as compared with those found in monocytes from normal individuals. Amounts of cell-associated IL-1 were high in patients with AIDS and in patients from Centers for Disease Control groups II and III. Serum-free culture for 24 h of monocytes from HIV-infected individuals in the absence of lipopolysaccharides (LPS) resulted in spontaneous release of IL-1 activity from the cells whereas no release occurred upon culture of normal cells. Stimulation of monocytes with LPS induced the release of IL-1 alpha and IL-1 beta from cells of infected patients. Only IL-1 beta was released from cells of normal individuals. Thus, circulating monocytes from HIV-infected patients are triggered to produce IL-1 in vivo. The present study also indicates that HIV infection is associated with an acquired defect in the intracellular processes regulating IL-1 secretion.
白细胞介素-1（IL-1）并非由正常人单核细胞持续产生。我们研究了来自HIV感染患者的未培养、未刺激的贴壁单核细胞中细胞相关IL-1的产生情况，这反映了体内细胞持续生成IL-1的过程。与正常个体的单核细胞相比，在HIV感染患者的单核细胞中发现了高水平的细胞相关IL-1活性以及细胞相关IL-1α和IL-1β抗原。在艾滋病患者以及疾病控制中心II组和III组的患者中，细胞相关IL-1的量很高。在无脂多糖（LPS）的情况下，对来自HIV感染个体的单核细胞进行24小时无血清培养，导致细胞自发释放IL-1活性，而正常细胞培养时则无释放。用LPS刺激单核细胞可诱导感染患者细胞释放IL-1α和IL-1β。正常个体的细胞仅释放IL-1β。因此，HIV感染患者的循环单核细胞在体内被触发产生IL-1。本研究还表明，HIV感染与调节IL-1分泌的细胞内过程中获得性缺陷有关。
