Liu Hua, Xu Xuan-Fu, Zhao Yan, Tang Mao-Chun, Zhou Ying-Qun, Lu Jie, Gao Feng-Hou
Department of Gastroenterology, The Tenth Hospital Affiliated to Tongji University, No. 301, Yanchang Road, 200072, Shanghai, China.
Tumour Biol. 2014 Dec;35(12):12157-63. doi: 10.1007/s13277-014-2521-9. Epub 2014 Aug 29.
Recent studies have shown that microRNAs, a class of small and noncoding RNA molecules, play crucial roles in the initiation and progression of pancreatic cancer. In the present study, the expression and roles of miR-191 were investigated. Through both gain-of function and loss-of function experiments, a pro-oncogenic function of miR-191 was demonstrated. At the molecular level, bioinformatic prediction, luciferase, and protein expression analysis suggested that miR-191 could inhibit protein levels of UPS10, which suppressed the proliferation and growth of cancer cells through stabilizing P53 protein. Collectively, these data suggest that miR-191 could promote pancreatic cancer progression through targeting USP10, implicating a novel mechanism for the tumorigenesis.
最近的研究表明，微小RNA（一类小的非编码RNA分子）在胰腺癌的发生和发展中起关键作用。在本研究中，对miR-191的表达及其作用进行了研究。通过功能获得和功能丧失实验，证实了miR-191具有促癌功能。在分子水平上，生物信息学预测、荧光素酶和蛋白质表达分析表明，miR-191可以抑制UPS10的蛋白质水平，而UPS10通过稳定P53蛋白来抑制癌细胞的增殖和生长。总的来说，这些数据表明miR-191可能通过靶向USP10促进胰腺癌进展，这意味着一种新的肿瘤发生机制。
