Tedesco Maybell, Felippe Mara Cristina Santos, Felippe Wilson Tadeu, Alves Ana Maria Hecke, Bortoluzzi Eduardo Antunes, Teixeira Cleonice Silveira
Department of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, CEP 88040-900, Brazil.
Microsc Res Tech. 2014 Dec;77(12):1015-22. doi: 10.1002/jemt.22430. Epub 2014 Sep 2.
This study evaluated the bond strength (BS) and the adhesive interface of four endodontic sealers to root canal dentine, before, and after immersion in phosphate buffered saline (PBS) to simulate an in vivo environment. Eighty roots were instrumented using ProTaper rotatory files, under irrigation with 17% EDTA and 1% NaOCl. Posteriorly were divided into four groups (n = 20) according to the sealer used: Endofill, AH Plus, Sealapex, and MTA Fillapex. Each group was divided into two subgroups (n = 10) and stored at 37°C immersed in water for 7 days and in PBS for 60 days. From each subgroup, 1 mm thick sections were obtained. One section of each region (coronal, middle, and apical) was submitted to the push-out test and failures were observed. Twelve sections of each subgroup (four from each region) were evaluated under SEM. Three-way ANOVA evaluation for BS showed significant differences between groups and regions (P < 0.0001), but not between subgroups (P > 0.05). AH Plus had significantly higher BS than the others sealers, regardless of the analyzed subgroup (Tukey's test, P < 0.5). The most common failures were adhesive to dentine and cohesive of the sealer. The SEM evaluation (Kruskal-Wallis, Mann-Whitney) showed homogeneous adhesive interface formed and sealer tags in all groups with significant statistical differences with AH Plus, regardless of PBS immersion. AH Plus was superior to the other sealers for both BS and quality of interface formation. Immersion in PBS did not interfere on BS or adhesive interface of the sealers tested.
本研究评估了四种根管封闭剂在模拟体内环境的磷酸盐缓冲盐水（PBS）中浸泡前后与根管牙本质的粘结强度（BS）和粘结界面。使用ProTaper旋转锉对80颗牙根进行预备，同时用17%乙二胺四乙酸（EDTA）和1%次氯酸钠（NaOCl）冲洗。之后，根据所使用的封闭剂将其分为四组（n = 20）：Endofill、AH Plus、Sealapex和MTA Fillapex。每组再分为两个亚组（n = 10），分别在37°C下于水中浸泡7天和在PBS中浸泡60天。从每个亚组中获取1毫米厚的切片。对每个区域（冠部、中部和根尖部）的一片切片进行推出试验，并观察失败情况。对每个亚组的12片切片（每个区域4片）进行扫描电子显微镜（SEM）评估。对粘结强度进行的三因素方差分析显示，组间和区域间存在显著差异（P < 0.0001），但亚组间无显著差异（P > 0.05）。无论分析的亚组如何，AH Plus的粘结强度均显著高于其他封闭剂（Tukey检验，P < 0.5）。最常见的失败类型是与牙本质的粘结性失败和封闭剂的内聚性失败。SEM评估（Kruskal-Wallis检验、Mann-Whitney检验）显示，所有组均形成了均匀的粘结界面和封闭剂标签，与AH Plus相比存在显著统计学差异，且不受PBS浸泡的影响。对于粘结强度和界面形成质量，AH Plus均优于其他封闭剂。浸泡在PBS中并未影响所测试封闭剂的粘结强度或粘结界面。
