Kebudi Benezra Mira, Schembri Wismayer Pierre, Camilleri Josette
Department of Endodontics, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey.
Department of Anatomy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
Sci Rep. 2017 Dec 20;7(1):17927. doi: 10.1038/s41598-017-17280-7.
In vitro material testing is undertaken by conducting a series of tests following procedures outlined in international standards. All material properties are measured in water; however biological behavior is undertaken in alternative media such as Dulbecco's modified eagle medium (DMEM) or simulated body fluid. The aim of this study was to characterize four dental root canal sealers and study their properties in different media. Four dental root canal sealers were assessed. They were characterized by a combination of techniques and the sealer properties were tested as specified by ISO 6876 (2012) and also in alternative media. The sealer biocompatibility was measured by cell function and proliferation assays of elutions. All sealers complied with ISO specifications. The material properties were effected by the type of soaking medium used and the surface micromorphology and elemental composition were dependent on the soaking solution type. Both BioRoot and MTA Fillapex showed cytotoxicity which reduced at higher dilutions. The material chemistry, presentation, environmental conditions and testing methodology used affected the sealer properties. Standards specific to sealer type are thus indicated. Furthermore the methodology used in the standard testing should be more relevant to clinical situations.
体外材料测试是按照国际标准中概述的程序进行一系列测试来开展的。所有材料特性均在水中测量；然而生物行为是在诸如杜尔贝科改良伊格尔培养基（DMEM）或模拟体液等替代介质中进行的。本研究的目的是对四种牙根管封闭剂进行表征，并研究它们在不同介质中的特性。对四种牙根管封闭剂进行了评估。通过多种技术相结合对它们进行表征，并且按照ISO 6876（2012）的规定以及在替代介质中测试封闭剂的特性。通过洗脱液的细胞功能和增殖测定来测量封闭剂的生物相容性。所有封闭剂均符合ISO规范。材料特性受所用浸泡介质类型的影响，并且表面微观形态和元素组成取决于浸泡溶液类型。BioRoot和MTA Fillapex均表现出细胞毒性，在较高稀释度下毒性降低。所用的材料化学、呈现方式、环境条件和测试方法会影响封闭剂的特性。因此表明了特定于封闭剂类型的标准。此外，标准测试中使用的方法应与临床情况更相关。
