Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2013 May;103(5):1141-8. doi: 10.1007/s10482-013-9896-2. Epub 2013 Feb 27.
A Gram-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, GGW-M5(T), was isolated from seawater on the southern coast in Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain GGW-M5(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain GGW-M5(T) belonged to the genus Glaciecola, joining the cluster comprising the type strains of G. agarilytica, G. arctica, G. chathamensis, G. mesophila, G. polaris and G. psychrophila, with which it exhibited 16S rRNA gene sequence similarity values of 95.9-96.7 %. Strain GGW-M5(T) exhibited sequence similarity values of 93.2-94.8 % to the type strains of the other Glaciecola species. Strain GGW-M5(T) contained Q-8 as the predominant ubiquinone and C16:1 ω7c and/or iso-C15:0 2-OH, C16:0 and C14:0 2-OH as the major fatty acids. Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GGW-M5(T) could be distinguished from other Glaciecola species. On the basis of the data presented, strain GGW-M5(T) is considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola aquimarina sp. nov. is proposed. The type strain is GGW-M5(T) (=KCTC 32108(T) = CCUG 62918(T)).
一株革兰氏阴性、需氧、非运动、卵形或杆状的细菌菌株 GGW-M5(T) 从韩国南部海岸的海水中分离得到，采用多相分类法研究了其分类地位。菌株 GGW-M5(T) 在 pH7.0-8.0、30°C 和 2%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 基因序列的邻接法系统发育树显示，菌株 GGW-M5(T) 属于 Glaciecola 属，与包括 G. agarilytica、G. arctica、G. chathamensis、G. mesophila、G. polaris 和 G. psychrophila 等模式菌株一起形成一个聚类，其 16S rRNA 基因序列相似性值为 95.9-96.7%。与其他 Glaciecola 种的模式菌株相比，菌株 GGW-M5(T) 的 16S rRNA 基因序列相似性值为 93.2-94.8%。菌株 GGW-M5(T) 含有 Q-8 作为主要的泛醌，C16:1 ω7c 和/或 iso-C15:0 2-OH、C16:0 和 C14:0 2-OH 作为主要的脂肪酸。主要的极性脂为磷脂酰甘油、磷脂酰乙醇胺和双磷脂酰甘油。DNA G+C 含量为 42.4mol%。表型特征的差异以及系统发育的独特性表明，菌株 GGW-M5(T) 可与其他 Glaciecola 种区分开来。基于所提供的数据，菌株 GGW-M5(T) 被认为代表了 Glaciecola 属的一个新种，建议将其命名为 Glaciecola aquimarina sp. nov.。模式菌株为 GGW-M5(T) (=KCTC 32108(T) = CCUG 62918(T))。
