Lu Xiaoling, Zhou Yanhua, Wu Tao, Hao Lei
Key Laboratory of Nutrition and Safety, Ministry of Education, Department of Food Sciences & Bioengineering, Tianjin University of Science & Technology, Tianjin 300457, PR China.
Food Funct. 2014 Nov;5(11):2892-7. doi: 10.1039/c4fo00391h.
This study investigated the ameliorative effect of black rice anthocyanin (BACN) in senescent mice induced by D-galactose. The male mice were randomly divided into five groups, namely, the normal group, the model group and dosage groups (15, 30 and 60 mg kg(-1) of BACN). The model group and three dosage groups were continuously injected subcutaneously with D-galactose. The results suggested that superoxide dismutase (SOD) and catalase (CAT) were significantly increased upon black rice anthocyanin treatment, while MDA and the activity of monoamine oxidase (MAO) significantly decreased. The expressions of superoxide dismutase genes (SOD1 and SOD2) in liver were up-regulated in black rice anthocyanin group, while the expression of the MAO-B gene was down-regulated. These findings demonstrated that the ameliorative effect of BACN might be achieved partly by altering endogenous antioxidant enzymatic and aging-related enzymatic activities and regulating SOD1, SOD2 and MAO-B gene expressions.
本研究调查了黑米花青素（BACN）对D-半乳糖诱导的衰老小鼠的改善作用。雄性小鼠被随机分为五组，即正常组、模型组和剂量组（BACN剂量分别为15、30和60 mg kg(-1)）。模型组和三个剂量组连续皮下注射D-半乳糖。结果表明，黑米花青素处理后超氧化物歧化酶（SOD）和过氧化氢酶（CAT）显著增加，而丙二醛（MDA）和单胺氧化酶（MAO）活性显著降低。黑米花青素组肝脏中超氧化物歧化酶基因（SOD1和SOD2）的表达上调，而MAO-B基因的表达下调。这些发现表明，BACN的改善作用可能部分是通过改变内源性抗氧化酶和衰老相关酶的活性以及调节SOD1、SOD2和MAO-B基因的表达来实现的。
