Wu Wenxiao, Li Jing, Chen Laizhong, Ma Zhao, Zhang Wei, Liu Zhenzhen, Cheng Yanna, Du Lupei, Li Minyong
Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
Anal Chem. 2014 Oct 7;86(19):9800-6. doi: 10.1021/ac502396g. Epub 2014 Sep 17.
Reactive oxygen species (ROS) often have significant roles in mediating redox modifications and other essential physiological processes, such as biological process regulation and signal transduction. Considering that H2O2 is a substantial member of ROS, detection and quantitation of H2O2 undertakes important but urgent responsibility. In this report, a bioluminescent probe for detecting H2O2 was well designed, synthesized, and evaluated. This probe was designed into three parts: a H2O2-sensitive aryl boronic acid, a bioluminescent aminoluciferin moiety, and a self-immolative linker. After extensive evaluation, this probe can selectively and sensitively react with H2O2 to release aminoluciferin. It should be pointed out that this probe is a potential bioluminescent sensor for H2O2 since it can provide a promising toolkit for real-time detection of the H2O2 level in vitro, in cellulo, and in vivo.
活性氧（ROS）在介导氧化还原修饰和其他重要生理过程（如生物过程调节和信号转导）中常常发挥重要作用。鉴于过氧化氢（H2O2）是ROS的重要成员，H2O2的检测和定量承担着重要但紧迫的责任。在本报告中，一种用于检测H2O2的生物发光探针被精心设计、合成和评估。该探针被设计为三个部分：一个对H2O2敏感的芳基硼酸、一个生物发光的氨基荧光素部分和一个自毁连接子。经过广泛评估，该探针能够选择性且灵敏地与H2O2反应以释放氨基荧光素。应当指出，该探针是一种潜在的H2O2生物发光传感器，因为它可为体外、细胞内和体内H2O2水平的实时检测提供一个有前景的工具包。
